PrimePCR™ ddPCR™ Expression Probe Assay: B4GALT1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0058258; exonic

 
Fluorophore:   FAM
List Price:    $195.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   dHsaCPE5045758
Chromosome Location:   9:33116091-33120539question
Amplicon Length:   114

Gene Information

This gene is one of seven beta-14-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta14 linkage to similar acceptor sugars: GlcNAc Glc and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity the beta4GalTs form four groups: beta4GalT1 and beta4GalT2 beta4GalT3 and beta4GalT4 beta4GalT5 and beta4GalT6 and beta4GalT7. This gene is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose <=> UDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing. Two transcripts which differ only at the 5' end with approximate lengths of 4.1 kb and 3.9 kb encode the same protein. The longer transcript encodes the type II membrane-bound trans-Golgi resident protein involved in glycoconjugate biosynthesis. The shorter transcript encodes a protein which is cleaved to form the soluble lactose synthase. [provided by RefSeq Jul 2008]

Gene Symbol:   B4GALT1
Gene Name:   UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
Aliases:   B4GAL-T1, CDG2D, DKFZp686N19253, GGTB2, GT1, GTB, MGC50983, beta4Gal-T1
RefSeq:   NC_000009.11 NG_008919.1 NT_008413.18
Ensembl:   ENSG00000086062
Entrez:   2683
UniGene:   Hs.272011
Chromosome Mapping:   9p13

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