This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
Info: HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0055361; exonic
Laminin an extracellular protein is a major component of the basement membrane. It is thought to mediate the attachment migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits alpha beta and gamma which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene. [provided by RefSeq Jul 2008]