This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
Info: FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0054152; exonic
The SPTLC3 gene encodes an isoform of the third subunit of serine palmitoyltransferase (SPT; EC 184.108.40.206) which catalyzes the rate-limiting step of the de novo synthesis of sphingolipids (Hornemann et al. 2006 [PubMed 17023427]). SPT contains 2 main subunits: the common SPTLC1 subunit (MIM 605712) and either SPTLC2 (MIM 605713) or its isoform SPTLC2L (SPTLC3) depending on the tissue in which biosynthesis occurs (Hornemann et al. 2006 [PubMed 17023427]). There are also 2 highly related isoforms of a third subunit SSSPTA (MIM 613540) and SSSPTB (MIM 610412) that confer acyl-CoA preference of the SPT enzyme and are essential for maximal enzyme activity (Han et al. 2009 [PubMed 19416851]).[supplied by OMIM Nov 2010]