PrimePCR™ ddPCR™ Expression Probe Assay: RASGRP4, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0054101; exonic

 
Fluorophore:   HEX
List Price:    $195.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   dHsaCPE5040783
Chromosome Location:   19:38901526-38901771question
Amplicon Length:   100

Gene Information

The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif a diacylglycerol-binding domain and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein and the RAS activation can be greatly enhanced by phorbol ester treatment which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Apr 2009]

Gene Symbol:   RASGRP4
Gene Name:   RAS guanyl releasing protein 4
Aliases:   Not Available
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000171777
Entrez:   115727
UniGene:   Hs.130434
Chromosome Mapping:   19q13.1

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