This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
Info: HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0053876; exonic
This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain two Raf-like Ras-binding domains (RBDs) and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding through its GoLoco domain to specific types of activated GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP) the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers forming inactive G-protein heterotrimers thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq Jul 2008]