This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
Info: HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0052852; exonic
This gene encodes a mitogen-responsive phosphoprotein. It is expressed in normal ovarian epithelial cells but is down-regulated or absent from ovarian carcinoma cell lines suggesting its role as a tumor suppressor. This protein binds to the SH3 domains of GRB2 an adaptor protein that couples tyrosine kinase receptors to SOS (a guanine nucleotide exchange factor for Ras) via its C-terminal proline-rich sequences and may thus modulate growth factor/Ras pathways by competing with SOS for binding to GRB2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2011]