PrimePCR™ ddPCR™ Expression Probe Assay: RDH8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0049715; exonic

Fluorophore:   HEX
List Price:    $201.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   dHsaCPE5033051
Chromosome Location:   19:10132420-10132563question
Amplicon Length:   114

Gene Information

All-trans-retinol dehydrogenase (RDH8) is a visual cycle enzyme that reduces all-trans-retinal to all-trans-retinol in the presence of NADPH (Rattner et al. 2000 [PubMed 10753906]). It is a member of the short chain dehydrogenase/reductase family and is located in the outer segments of photoreceptors; hence it is also known as photoreceptor retinol dehydrogenase. It is important in the visual cycle by beginning the rhodopsin regeneration pathway by reducing all-trans-retinal the product of bleached and hydrolysed rhodopsin (Rando 2001 [PubMed 11710234]). This is a rate-limiting step in the visual cycle (Saari et al. 1998 [PubMed 9667000]).[supplied by OMIM Mar 2008]

Gene Symbol:   RDH8
Gene Name:   retinol dehydrogenase 8 (all-trans)
Aliases:   PRRDH, SDR28C2
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000080511
Entrez:   50700
UniGene:   Hs.675522
Chromosome Mapping:   19p13.2

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