This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
Info: FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0025327; Exonic
This gene encodes D-2hydroxyglutarate dehydrogenase a mitochondrial enzyme belonging to the FAD-binding oxidoreductase/transferase type 4 family. This enzyme which is most active in liver and kidney but also active in heart and brain converts D-2-hydroxyglutarate to 2-ketoglutarate. Mutations in this gene are present in D-2-hydroxyglutaric aciduria a rare recessive neurometabolic disorder causing developmental delay epilepsy hypotonia and dysmorphic features. [provided by RefSeq Jul 2008]