This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
Info: FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0024891; Exonic
This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone cornea dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV Ehlers-Danlos syndrome type VIIB recessive Ehlers-Danlos syndrome Classical type idiopathic osteoporosis and atypical Marfan syndrome. Symptoms associated with mutations in this gene however tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts resulting from the use of alternate polyadenylation signals have been identified for this gene. [provided by R. Dalgleish Feb 2008]