PrimePCR™ ddPCR™ Copy Number Assay: MAPT, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; 69nt amplicon; intronic; HEX version of MAPT (dHsaCP1000170)

 
Fluorophore:   HEX
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2507095
Chromosome Location:   hg1917:44095861-44095983question
Amplicon Length:   69
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex regulated alternative splicing giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease Pick's disease frontotemporal dementia cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq Jul 2008]

Gene Symbol:   MAPT
Gene Name:   microtubule-associated protein tau
Aliases:   DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU
RefSeq:   NC_000017.10 NT_010783.15 NT_167251.1 NG_007398.1
Ensembl:   ENSG00000186868
Entrez:   4137
UniGene:   Hs.101174
Chromosome Mapping:   17q21.1

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

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