PrimePCR™ ddPCR™ Copy Number Assay: HMOX2, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; 76nt amplicon; intronic; HEX version of HMOX2 (dHsaCP1000204)

 
Fluorophore:   HEX
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2507064
Chromosome Location:   hg1916:4557061-4557183question
Amplicon Length:   76
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

Heme oxygenase an essential enzyme in heme catabolism cleaves heme to form biliverdin which is subsequently converted to bilirubin by biliverdin reductase and carbon monoxide a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Alternative splice variants encoding the same protein have been identified at this locus. [provided by RefSeq Jul 2008]

Gene Symbol:   HMOX2
Gene Name:   heme oxygenase (decycling) 2
Aliases:   HO-2
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000103415
Entrez:   3163
UniGene:   Hs.284279
Chromosome Mapping:   16p13.3

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to TERT reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to TERT reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

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