PrimePCR™ ddPCR™ Mutation Assay: ABL1 WT for p.E255K, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   61nt amplicon; exonic

 
Fluorophore:   HEX
List Price:    $395.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Mutation Detection
Unique Assay ID:   dHsaCP2506765
Chromosome Location:   9:133738302-133738424question
Amplicon Length:   61
Cosmic ID   COSM12573
Nucleotide Mutation Detection:   WT for c.763G>A
Amino Acid Change:   WT for p.E255K
Restriction Enzymes:   MseI

Gene Information

The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation cell division cell adhesion and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq Jul 2008]

Gene Symbol:   ABL1
Gene Name:   c-abl oncogene 1, non-receptor tyrosine kinase
Aliases:   ABL, JTK7, bcr/abl, c-ABL, p150, v-abl
RefSeq:   NC_000009.11 NT_035014.4 NG_012034.1
Ensembl:   ENSG00000097007
Entrez:   25
UniGene:   Hs.431048
Chromosome Mapping:   9q34.1

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample synthetic dsDNA (mut), Promega female human genomic DNA (WT)

%Fractional Abundance
Dilution series of mutant DNA in ~40,000 copies of wild-type DNA background. Technical replicates are offset for visualization.

ddPCR Amplitude Scatter Plot (wild type + mutant)
Single-well data for mutant DNA spiked into wild-type DNA (~0.1%). Mutation assay (FAM, Channel 1) was duplexed with the corresponding wild-type reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot (wild type + mutant)

ddPCR Amplitude Scatter Plot (wild type)
Single-well data for wild-type DNA (no mutant). Mutation assay (FAM, Channel 1) was duplexed with the corresponding wild-type reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot (wild type)

Number Description Download
10033487 ddPCR™ Mutation Detection Assays Product Insert, Ver C Click to download
10033173 ddPCR™ Copy Number Variation Assays Product Insert, Ver C Click to download
10041702 PrimePCR™ ddPCR™ Gene Expression Assays (EvaGreen) Product Insert, Rev A Click to download
10047489 ddPCR™ Mutation Detection Assays Product Insert, Ver B Click to download
10048730 PrimePCR™ ddPCR™ Gene Expression Probe Assays Product Insert, Rev A Click to download
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