PrimePCR™ ddPCR™ Copy Number Assay: MELK, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; 70nt amplicon; exonic; HEX version of MELK (dHsaCP2500427)

 
Fluorophore:   HEX
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2506612
Chromosome Location:   hg199:36677188-36677310question
Amplicon Length:   70
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

Description Not Available

Gene Symbol:   MELK
Gene Name:   maternal embryonic leucine zipper kinase
Aliases:   HPK38, KIAA0175
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000165304
Entrez:   9833
UniGene:   Hs.184339
Chromosome Mapping:   9p13.2

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download