PrimePCR™ ddPCR™ Copy Number Assay: WSB1, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Centromeric 17q Assay (17q11.1); FAM; 63nt amplicon; crosses exon-intron junction; FAM version of WSB1 (dHsaCP2506685)

 
Fluorophore:   FAM
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2506294
Chromosome Location:   hg1917:25628887-25629009question
Amplicon Length:   63
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

This gene encodes a member of the WD-protein subfamily. This protein shares a high sequence identity to mouse and chick proteins. It contains several WD-repeats spanning most of the protein and an SOCS box in the C-terminus. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   WSB1
Gene Name:   WD repeat and SOCS box containing 1
Aliases:   SWIP1, WSB-1
RefSeq:   NC_000017.10 NT_010799.15
Ensembl:   ENSG00000109046
Entrez:   26118
UniGene:   Hs.446017
Chromosome Mapping:   17q11.1

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download