PrimePCR™ ddPCR™ Copy Number Assay: CEBPD, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Centromeric 8q Assay (8q11.21); FAM; 61nt amplicon; exonic; FAM version of CEBPD (dHsaCP2506690)

 
Fluorophore:   FAM
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2506270
Chromosome Location:   hg198:48649940-48650062question
Amplicon Length:   61
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-alpha. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and may be involved in the regulation of genes associated with activation and/or differentiation of macrophages. The cytogenetic location of this locus has been reported as both 8p11 and 8q11. [provided by RefSeq Sep 2010]

Gene Symbol:   CEBPD
Gene Name:   CCAAT/enhancer binding protein (C/EBP), delta
Aliases:   C/EBP-delta, CELF, CRP3, NF-IL6-beta
RefSeq:   NC_000008.10 NT_008183.19
Ensembl:   ENSG00000221869
Entrez:   1052
UniGene:   Hs.440829
Chromosome Mapping:   8p11.2-p11.1

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download