PrimePCR™ ddPCR™ Copy Number Assay: WT1, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   FAM; 67nt amplicon; exonic; FAM version of WT1 (dHsaCP2506643)

 
Fluorophore:   FAM
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2500489
Chromosome Location:   hg1911:32410577-32410699question
Amplicon Length:   67
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern with biallelic and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq Oct 2010]

Gene Symbol:   WT1
Gene Name:   Wilms tumor 1
Aliases:   AWT1, GUD, NPHS4, WAGR, WIT-2, WT33
RefSeq:   NC_000011.9 NT_009237.18 NG_009272.1
Ensembl:   ENSG00000184937
Entrez:   7490
UniGene:   Hs.591980
Chromosome Mapping:   11p13

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download