PrimePCR™ ddPCR™ Copy Number Assay: SNCA, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   FAM; 63nt amplicon; crosses exon-intron junction; FAM version of SNCA (dHsaCP2506564)

 
Fluorophore:   FAM
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2500470
Chromosome Location:   hg194:90757827-90757949question
Amplicon Length:   63
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

Alpha-synuclein is a member of the synuclein family which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene. [provided by RefSeq Mar 2009]

Gene Symbol:   SNCA
Gene Name:   synuclein, alpha (non A4 component of amyloid precursor)
Aliases:   MGC110988, NACP, PARK1, PARK4, PD1
RefSeq:   NC_000004.11 NG_011851.1 NT_016354.19
Ensembl:   ENSG00000145335
Entrez:   6622
UniGene:   Hs.21374
Chromosome Mapping:   4q21

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download