This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
Info: 91nt amplicon; exonic
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway which affects cell division differentiation and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome a disease characterized by heart defects mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers including non-Hodgkin lymphoma colorectal cancer malignant melanoma thyroid carcinoma non-small cell lung carcinoma and adenocarcinoma of lung. A pseudogene which is located on chromosome X has been identified for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Dilution series of mutant DNA in ~40,000 copies of wild type DNA background.
ddPCR Amplitude Scatter Plot (wild type + mutant)
Single-well data for mutant DNA spiked into wild-type DNA (~0.1%). Mutation assay (FAM, Channel 1) was duplexed with the corresponding wild-type reference assay (HEX, Channel 2).
ddPCR Amplitude Scatter Plot (wild type)
Single-well data for wild-type DNA (no mutant). Mutation assay (FAM, Channel 1) was duplexed with the corresponding wild-type reference assay (HEX, Channel 2).