PrimePCR™ ddPCR™ Copy Number Assay: EEF1A2, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   FAM: 65nt amplicon; exonic

 
Fluorophore:   FAM
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP1000412
Chromosome Location:   20:62121882-62122004question
Amplicon Length:   65
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain heart and skeletal muscle and the other isoform (alpha 1) is expressed in brain placenta lung liver kidney and pancreas. This gene may be critical in the development of ovarian cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2008]

Gene Symbol:   EEF1A2
Gene Name:   eukaryotic translation elongation factor 1 alpha 2
Aliases:   EEF1AL, EF-1-alpha-2, EF1A, FLJ41696, HS1, STN, STNL
RefSeq:   NC_000020.10 NT_011333.6
Ensembl:   ENSG00000101210
Entrez:   1917
UniGene:   Hs.433839
Chromosome Mapping:   20q13.3

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX100
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download