PrimePCR™ ddPCR™ Copy Number Assay: JUN, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   FAM: 120nt amplicon; exonic

 
Fluorophore:   FAM
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP1000030
Chromosome Location:   1:59247320-59247442question
Amplicon Length:   120
Reference Assays:   , , , , , , ,
Restriction Enzymes:   HaeIII

Gene Information

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31 a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq Jul 2008]

Gene Symbol:   JUN
Gene Name:   jun proto-oncogene
Aliases:   AP-1, AP1, c-Jun
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000177606
Entrez:   3725
UniGene:   Hs.696684
Chromosome Mapping:   1p32-p31

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX100
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download