PrimePCR™ ddPCR™ Copy Number Assay: CDKN1A, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

 
Fluorophore:   FAM
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Assay Information

Technology:   ddPCR
Assay Type   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP1000015
Chromosome Location:   6: 36653944-36654066question
Amplicon Length:   94
Reference Assays   AP3B1(dHsaCP1000001) , EIF2C1(dHsaCP1000002) , RPP30(dHsaCP1000485) , TERT(dHsaCP1000100)
Restriction Enzymes   HaeIII

Gene Information

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53 through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA) a DNA polymerase accessory factor and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases which thus leads to a dramatic activation of CDK2 and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq Nov 2010]

Gene Symbol:   CDKN1A
Gene Name:   cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Aliases:   CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1
RefSeq:   NC_000006.11 NG_009364.1 NT_007592.15
Ensembl:   ENSG00000124762
Entrez:   1026
UniGene:   Hs.370771
Chromosome Mapping   6p21.2

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX100
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download
10033487 PrimePCR ddPCR Mutation Detection Assays Product Insert, Rev B Click to download
10033173 PrimePCR ddPCR Copy Number Variation (CNV) Assays Product Insert, Rev B Click to download
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