Normalization of Real-Time PCR Fluorescence Data with ROX Passive Reference Dye
Which real-time PCR supermixes and one-step RT-PCR kits contain ROX universal passive reference dye?
The following products contain ROX universal passive reference dye:
Supermixes for PCR and Real-Time PCR
- iTaq™ Universal SYBR® Green Supermix
- iTaq Universal Probes Supermix
- SsoAdvanced™ Universal SYBR® Green Supermix
- SsoAdvanced Universal Probes Supermix
One-Step RT-PCR Kits
See Normalization of Real-Time PCR Fluorescence Data with ROX Passive Reference Dye in our Applications and Technologies pages to learn why passive reference dyes are required for data normalization in some real-time PCR systems and not others. Although Bio-Rad real-time PCR detection systems do not require ROX for internal normalization, Bio-Rad’s universal real-time PCR supermixes are designed for high performance on all real-time PCR detection systems, both systems requiring ROX normalization and ROX-independent systems.
View our Understanding Real-Time Supermixes tutorial to learn more about qPCR data analysis and how to compare supermix performance.