How will Sso7d fusion polymerase improve my qPCR assays?

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Normalization of Real-Time PCR Fluorescence Data with ROX Passive Reference Dye


Question:

How will Sso7d fusion polymerase improve my qPCR assays?


Answer:

By stabilizing the polymerase-template complex, Sso7d fusion polymerase provides a number of advantages over ordinary DNA polymerases. The tighter association between the enzyme and the template strand results in increased processivity, increased read-through in regions of GC-rich sequence or strong secondary structure, longer products, and higher yields; additionally Sso7d fusion polymerase displays superior tolerance to PCR inhibitors.

View our Understanding Real-Time Supermixes tutorial to learn more about qPCR data analysis and how to compare supermix performance.


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