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ProteOn™ XPR36 Protein Interaction Array System


Question:

What are the ProteOn HTG and HTE sensor chips?


Answer:

The HTG and HTE sensor chips feature a novel tris-NTA (3-NTA) surface for improved capturing of histidine-tagged proteins. The functional group tris-NTA, unique to the ProteOn HTG and HTE sensor chips, has a significantly higher binding stability compared to that of the traditional mono-NTA (NTA) surface. This results in minimal ligand drift and improves sensorgram baseline stability. Bio-Rad offers two ProteOn sensor chips for various histidine-tagged protein applications: HTG for compact-density (large molecule) applications and HTE for high-density (small molecule) applications. Both the HTG and HTE sensor chips allow easy surface regeneration, chip reuse, and capture of histidine-tagged proteins directly from crude samples.


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