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ProteOn™ XPR36 Protein Interaction Array System


Question:

How many times is it possible to regenerate a sensor chip surface?


Answer:

The extent of regeneration depends on the immobilized protein ligand. In the case of small molecule screening, running buffer is often the regeneration solution. Therefore, the protein ligand remains quite stable and active for multiple rounds of analyte injections. However, when an acid, base, or detergent is required for regeneration, the ligand may lose its activity and using a positive control is recommended to monitor ligand activity.


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