Chromatography Systems


The pump cannot maintain steady backpressure. In some cases, one pump head works and the other does not.


  • It’s likely that the check valves are not working properly. The valves should be removed and cleaned or replaced
  • The pump may not be completely primed. The buffer reservoir should be set above the pump inlet and the buffer should be forced through the pump with a syringe. In some cases, using an alcohol such as ethanol or isopropanol will remove air more quickly since the air will dissolve into the alcohol more easily
  • Occasionally, some small backpressure is required after the pump to properly seat the outlet check valve. A flow restrictor is used for this purpose

Get Help
Service and Support

Technical Support: 1-800-4-BIORAD (1-800-424-6723)

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