Model 550, Benchmark™, Benchmark™ Plus, Model 680 Series Microplate Readers

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Model 550, Benchmark™, Benchmark™ Plus, Model 680 Series Microplate Readers


Question:

How can I quantitate DNA using the Model 550, Benchmark™, Benchmark™ Plus, or Model 680 series microplate readers?


Answer:

These microplate readers are capable of spectrophotometric analyses above the UV wavelengths only. They do not detect fluorescence. Therefore, only colorimetric DNA quantitation methods may be used.

The diphenylamine method in the following reference measures DNA absorbance at 570 nm; see Analytical Biochemistry (1983) 131: 394–396. The described method suggests not using a reference filter to adjust for plate variations because the chromophore absorbs in the 350–670 nm range.


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