Criterion Stain Free™ Gel Imaging System
Criterion Stain Free™ Gel Imaging System
Can Criterion Stain Free™ gels be used with blotting applications?
Criterion Stain Free gels are compatible with western blotting and provide a quick assessment of protein-transfer efficiency. After blotting, both the gel and the blot can be imaged to assess how much protein remains in the gel and how much was transferred to the membrane. There is no need for post-transfer staining. The images give quantitative data detailing any uneven transfer across the blot, which can then be used to achieve more precise quantitative immunodetection.
The fluorescent staining of proteins in Criterion Stain Free gels is the product of a reaction (activation) with tryptophan (Trp) residues in the proteins. Since Trp is only about 1–2% of the residues in most proteins, most antibodies will not be affected by the Trp modification. A chemiluminescent signal may have a slightly reduced band intensity but the proteins are still detected.
Polyclonal antibody detection is not affected by activation. Detection using monoclonal antibodies may be unaffected or may show decreased signal if the epitopes of the sample protein contain Trp residues. If the epitopes do not contain Trp, there should be no effect on the binding of monoclonal antibodies.
For more information on blotting applications, see Stain-Free Approach to Western Blotting Applications, bulletin #RP0051.
I cannot find the Certificate of Analysis I am looking for. Help!
When using our online Certificate of Analysis search tool, keep the following in mind:
- Make sure you entered the correct catalog number and lot/control number in the search fields
- If you have a kit, try looking up the Certificate of Analysis using the kit information as well as the information for the individual components
- The Certificate of Analysis that you are looking for may not be available via the website. In that case, you can contact your Bio-Rad representative or use the Request Form
If you cannot find the Certificate of Analysis you are looking for, please Contact Us.
Where can I find the catalog number, SKU number, or product number?
The catalog number, SKU number, or product number is printed on your product label. See the sample label below for the location of this information.
Where can I find the lot or control number?
The lot number or control number (one or the other, not both) is printed on your product label. See the sample label below for the location of this information.
Why are Certificates of Analysis not on the Documents tab?
Certificates of Analysis are associated not only with a product, but also with a specific lot of that product. For a given product, there may be several Certificates of Analysis, especially if it is an older product line and has had several lots produced over the years. Using the Certificate of Analysis search tool, you can enter the catalog number and lot/control number for the specific product you have on hand and download the exact Certificate of Analysis you need.
I have a batch number, not a lot/control number. How do I find the Certificate of Analysis for my item?
You can use the batch number in place of the lot/control number. Use the Certificate of Analysis search tool, entering the catalog number as usual and the batch number where the lot/control number would go.
Can I get a Certificate of Analysis even if my item is expired?
Yes. While we do periodically remove Certificates of Analysis for site maintenance purposes, we typically keep them available well beyond a product's expiration date.
Why does it say "Product Name Not Found" for a Certificate of Analysis listed in the search results?
There are Certificates of Analysis that correspond to products that are either discontinued or simply not available on the website. In these situations, the Certificate of Analysis is available for download, but other product details, such as the product name, are not.
Why does it say "Not Available" for a Certificate of Analysis listed in the search results?
In this case, you have entered a valid catalog number and lot/control number and we have located the Certificate of Analysis. However, for some reason, the actual file itself is unavailable.
Please use the Request Form here or contact your local Bio-Rad Representative so that we can send you the Certificate of Analysis.
All Bio-Rad products are guaranteed to meet the specifications listed in our catalog. Should a Bio-Rad branded product fail to meet specifications during its warranty period, it shall be repaired or replaced at Bio-Rad's discretion. Warranty periods are listed on quotations for specific products; however, instruments generally receive 1 year of warranty coverage from the date of shipment or installation. Instrument warranties do not include consumable parts, such as lamps, lasers, and platinum wire. Repairs have different warranty periods. Chemicals and other consumables are warranted through their expiration date, or for 1 year from shipment if no expiration date is indicated. Non-Bio-Rad branded products, such as computers and computer peripherals, are covered by the original equipment manufacturer's warranty. Warranties are not transferable from the original purchaser. Warranty coverage may be void if the product is moved outside of the country where the product was originally purchased. An optional extended warranty program is available. For information, call 1-800-4BIORAD, and select Inside Sales.
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