Bio-Plex® Cytokine Assays

Print

Bio-Plex® Cytokine Assays


Question:

Is Tris/glycine buffer compatible with Bio-Plex® cytokine assays?


Answer:

Tris/glycine buffer is not compatible with Bio-Plex cytokine assays. The cytokines in both the samples and standards already generally exist in low concentrations. Low-pH Tris/glycine buffer degrades cytokines so they are no longer in native form. Our antibodies are directed to native, non-denatured proteins, so you will get a weak signal if using Tris/glycine buffer.

In addition, if you dilute the standards in Tris/glycine buffer without using bovine serum albumin (BSA) or other carrier proteins, the proteins in the standards can stick to the walls of the wells, resulting in a low signal.


Get Help
Service and Support

Technical Support: 1-800-4-BIORAD (1-800-424-6723)

Bio-Rad ISO Certificates

Download Bio-Rad's ISO Certificates:

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.