Electrophoresis SDS-PAGE Standards

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Electrophoresis SDS-PAGE Standards


Question:

What sample buffer should I use with the unstained SDS-PAGE standards?


Answer:

Use Laemmli sample buffer (1610737), or an equivalent, for SDS-PAGE standards (1610303, 1610304, and 1610317).

The composition of the Laemmli sample buffer is 2% w/v SDS, 5% v/v β-mercaptoethanol, 62.5 mM Tris-HCl, pH 6.8, 10% v/v glycerol, and add (in a 1:20 ratio with the buffer) 0.01% w/v Bromophenol Blue. β-mercaptoethanol is volatile and should be added to the sample stock solution just before use. If there is not enough reductant in the sample buffer, the soybean trypsin inhibitor and ovalbumin bands of the standards may run as doublets.

Note: The polypeptide SDS-PAGE ~1,400–~26,600 MW range standards, (1610326), uses a Tris-Tricine buffer. Please see the Instruction Manual forPolypeptide SDS-PAGE Molecular Weight Standards.


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