Technical Support FAQ

Print

Technical Support FAQ


Question:

What buffer can I use for western blotting that does not contain glycine? I want to sequence my protein after blotting.


Answer:

You can use Tris/CAPS buffer, 60 mM Tris, 40 mM CAPS, pH 9.6, with or without SDS and/or methanol. (Do not adjust pH with acid or base.) 10 X CAPS Buffer (1610778) is available.

This buffer is especially effective with the Trans-Blot® SD Semi-Dry Transfer Cell. It is also a good buffer for the Trans-Blot Cell. Refer to Bulletin 2134, "Increased Transfer Efficiency Using a Discontinuous Buffer System", for the use of this buffer with the Trans-Blot SD Cell.

Another buffer you can use is 10 mM NaHCO3 , 3 mM Na2CO3 (20% methanol), pH 9.9 (do not adjust pH). This buffer is volatile, and must be prepared from the solid carbonates just before use.


Get Help
Service and Support

Technical Support: 1-800-4-BIORAD (1-800-424-6723)

Bio-Rad ISO Certificates

Download Bio-Rad's ISO Certificates:

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.