Technical Support FAQ

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Technical Support FAQ


Question:

Can I use SYBR® Green I as a fluorescent stain with the Gel Doc 1000 system?


Answer:

Yes, the Gel Doc 1000 system will work with SYBR® Green. SYBR® Green I has an optimal excitation wavelength at 254 nm and optimally fluoresces at 520 nm. With the Gel Doc 1000 system, at 302 nm transillumination, sensitivity is approximately 70 pg of dsDNA per band. If optional 254 nm transillumination is used, sensitivity is approximately 35 pg of dsDNA per band.

254 nm bulbs can be purchased using catalog number 100–1252.


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