Technical Support FAQ

Print

Technical Support FAQ


Question:

How can I convert my DGGE conditions to TTGE conditions?


Answer:

The condtions for DGGE (denaturing gradient gel electrophoresis) are not directly convertible to TTGE (temporal temperature gradient electrophoresis), but you can use them as a starting point for optimization.

From DGGE, you can get an idea of the percent denaturant required to get melting. This percent denaturant can then be used as a starting point for a TTGE gel. The temperature used for DGGE can be used as the midpoint in a temperature gradient of 2 degrees below to 2 degrees above this temperature.

Bulletin 2408 contains details on using DGGE data to convert to TTGE conditions.


Get Help
Service and Support

Technical Support: 1-800-4-BIORAD (1-800-424-6723)

Bio-Rad ISO Certificates

Download Bio-Rad's ISO Certificates:

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.