Technical Support FAQ


Technical Support FAQ


How can I convert my DGGE conditions to TTGE conditions?


The condtions for DGGE (denaturing gradient gel electrophoresis) are not directly convertible to TTGE (temporal temperature gradient electrophoresis), but you can use them as a starting point for optimization.

From DGGE, you can get an idea of the percent denaturant required to get melting. This percent denaturant can then be used as a starting point for a TTGE gel. The temperature used for DGGE can be used as the midpoint in a temperature gradient of 2 degrees below to 2 degrees above this temperature.

Bulletin 2408 contains details on using DGGE data to convert to TTGE conditions.

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