Technical Support FAQ

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Ready Gel Precast Gels for Mini-PROTEAN® Systems


Question:

What is the composition of the 10x Tris/glycine buffer, catalog #161-0734?


Answer:

10x Tris/glycine buffer, available in a 1 liter bottle (catalog #161-0734) or a 5 liter cube (catalog #161-0771), consists of the following:

250 mM Tris
1.92 M Glycine

Dilute 1:10 with purified water for use. Be sure to mix the diluted buffer well before pouring it into the electrophoresis cell.

Final 1x concentrations are:
25 mM Tris
192 mM Glycine
pH 8.3

This buffer is used in native PAGE applications and for western blotting (electrophoretic transfer).


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