Technical Support FAQ

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Technical Support FAQ


Question:

How much DTT can be used in the Affi-Gel® Hz Coupling Buffer?


Answer:

1 mM DTT (1610611) can be used in the Affi-Gel Hz Coupling Buffer (1536054) when coupling antibodies to Affi-Gel Hz Hydrazide Gel for custom immunoaffinity separations. DTT is a very polar molecule and will cleave the n-hydroxysuccinimide from the bead, so a 1 mM DTT concentration should not be exceeded.


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