Technical Support FAQ

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Technical Support FAQ


Question:

Do you have any references explaining mutation detection technology? Do you have any references that cite the DGene or the DCode instruments?


Answer:

References explaining and validating mutation detection technology:
1) Ravnik-Glavac, Metka, et al, "Sensitivity of Single-Strand Conformation Polymorphism (SSCP) and Heteroduplex Method for Mutation Detection in the Cystic Fibrosis Gene," Hum. Mol. Genetics 3: 801-807 (1994).
2) Hayashi, Kenshi, "PCR-SSCP: A Simple and Sensitive Method for Detection of Mutations in the Genomic DNA," PCR Methods and Applications 1: 34-38 (1991).
3) Yoshino, Kenji, et al, "Temperature Sweep Gel Electrophoresis: A Simple Method to Detect Point Mutations," Nucleic Acids Res 19: 3153 (1991)
4) Orita, Masato, et al, "Detection of Polymorphisms of Human DNA by Gel Electrophoresis as Single-Strand Conformation Polymorphisms (SSCP), Proc. Natl. Acad. Sci. 86: 2766-2770 (1989)
5) Borresen, Anne-Lise, et al, "Constant Denaturant Gel Electrophoresis as a Rapid Screening Technique for p53 Mutations," Proc. Natl. Acad. Sci. 88: 8405-8409 (1991)
6) Fischer, S.G., and L.S. Lerman, "DNA Fragments Differing by Single Base-Pair Substitutions are Separated in Denaturing Gradient Gels: Correspondence with Melting Theory," Proc. Natl. Acad. Sci. 80: 1579-1583 (1983)
7) Hayashi, Kenshi, kand D. W. Yandell, "How Sensitive Is PCR-SSCP?" Hum. Mutation 2: 338-346 (1993)
8) Fodde, Riccardo, and Minoque Losekoot, "Mutation Analysis by Denaturing Gradient Gel Electrophoresis (DGGE)", Chapter 19 in Technologies for Detection of DNA Damage and Mutations, G.P. Pfeifer, ed, Plenus Press, New York, 1996
9) Sekiya, Takao, "Single-Strand Conformation Polymorphism Analysis," Chapter 21 in Technologies for Detection of DNA Damage and Mutations, G.P. Pfeifer, ed, Plenus Press, New York, 1996
Citations Citing the DGene or DCode instruments:
1) Borresen, Anne-Lise, "Constant Denaturant Gel Electrophoresis (CDGE) in Mutation Screening," Chapter 20 in Technologies for Detection of DNA Damage and Mutations, G.P. Pfeifer, ed, Plenum Press, New York, 1996
2) Short, Steven M., and Curtis A. Suttle, "Denaturing Gradient Gel Electrophoresis Resolves Virus Sequences Amplified with Degenerate Primers," BioTechniques 28: 20-26 (2000)
3) Miller, Kristina, et al, "Denaturing Gradient Gel Electrophoresis (DGGE): A Rapid and Sensitive Technique to Screen Nucleotide Sequence Variation in Populations," Biotechniques 27: 1016-1030 (1999)


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