Nitrocellulose membranes, PVDF membranes, low-fluorescence membranes, and filter paper for western blotting applications are available in various formats including precut sizes that match standardgel sizes.
Bio-Rad's PVDF membranes are available in rolls, precut sheets, or convenient membrane/filter paper sandwiches.
Detection of proteins blotted on low fluorescence PVDF membranes using a variety of fluorescent-conjugated secondary antibodies. Albumin-depleted human serum samples spiked with different amounts of STI were loaded on a Criterion™ Tris-HCl 4–20% gel. The amount of STI per lane is 250, 83, 28, 9, and 3 ng per lane. Rabbit anti-STI was used as the primary antibody and a variety of fluorescent-conjugated anti-rabbit secondary antibodies were used. Membranes were scanned using a VersaDoc™ MP 5000 system.
Bio-Rad's Immun-Blot PVDF membrane is ideal for chemiluminescent and colorimetric western blots. This PVDF membrane retains target protein very strongly while reducing nonspecific protein binding.
Bio-Rad's Sequi-Blot PVDF membrane delivers outstanding performance in protein sequencing, providing the binding capacity to sequence even low-abundance samples.
Bio-Rad's nitrocellulose is a proven medium for western, northern, and Southern blotting. The 0.45 µm pore size is recommended for most analytical blotting including protein, ssDNA, and RNA transfers.
Bio-Rad nitrocellulose membrane with a 0.2 µm pore size is optimized for western, northern, and Southern blotting of nucleic acids and low molecular weight proteins.
Made of pure nitrocellulose cast on an inert synthetic support, this 0.45 µm nitrocellulose membrane is a solid support for nucleic acid and protein applications that can withstand multiple reprobing.
This pure nitrocellulose 0.2 µm membrane backed by an inert synthetic support is ideal for nucleic acid and protein applications involving multiple reprobing.
Available in a range of thicknesses, Bio-Rad's blot absorbent filter papers are high-quality, 100% cotton fiber papers for use in electrophoresis and blotting applications.
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