The cartridges prepacked with Bio-Rad's CHT ceramic hydroxyapatite and CFT ceramic fluoroapatite media are convenient for evaluating performance of the different apatite media for a new application.
What is CHT Ceramic Hydroxyapatite Video
F3GA and DEAE
* Purification capacity based on bulk support; check individual instruction manuals for run conditions and specifications.** Column volume.
To read more click here
* Flow rate = 150 cm/hr. ** Flow rate = 300 cm/hr.
Workflow for mAb purification with UNOsphere SUPrA affinity, UNOsphere Q, and CHT Type 1 media.
Monoclonal Antibody Purification Using UNOsphere SUPrA Media
The first step in purification of an important class of therapeutic proteins, the polyclonal or monoclonal antibodies (mAbs), is their capture from plasma or tissue culture supernatants. Protein A–based media are by far the most common class of affinity products used for this purpose. They bind with high affinity to the Fc region of most subclasses of antibodies and are one of the standard tools used in antibody capture and purification. In combination with ion exchange and ceramic hydroxypatite chromatography, protein A–based media have been successfully used in the large-scale purification of numerous licensed mAb drugs.
CHT Packing Guidelines Video
How to pack CHT in a Bio-Rad InPlace column video
Advances in Separation & Purification: Purifying Monoclonal Antibodies
The affinity capture paradigm that dominates industrial IgG purification has proven unsuitable for IgMs because, in most cases, they are affected adversely by harsh elution conditions. The large size of IgMs is also a challenge because it limits the operating conditions and performance of traditional porous-particle-based chromatography media. This article describes how these challenges can be overcome with available technology to develop effective manufacturing procedures for IgM monoclonal antibodies.
Purification of IgG using CHT Ceramic Hydroxyapatite. Read more.
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