These rigid, macroporous, hydrophilic anion exchange resins meet the demands of process-scale applications. Chemically and mechanically stable, these resins perform best at low and medium pressures.
Dynamic protein binding capacity as a function of linear flow rate. A 100 ml sample (3 mg/ml) of BSA was run on a 1 x 10 cm (8 ml) column at the indicated flow rates. The buffer was 50 mM Tris-HCI, pH 8.3. Elution was with 1.0 M NaCl.
To read more click here
The Macro-Prep DEAE derivative has high dynamic binding capacity and exhibits consistently high resolution even at high flow rates and high sample loads. A sample containing 3 mg/ml BSA was separated on a 1 x 10 (3 ml) Macro-Prep DEAE column. Sample was loaded onto the column in 10 mM Tris-HCI, pH 7.6 (buffer A) and was eluted in a M NaCl in buffer A. Sample was applied until the eluting buffer had an A280 value 50% of the incoming sample.
The effect of flow rate on separation and peak symmetry. A 5 ml sample containing 3.65 mg/ml each myoglobin, conalbumin, BSA, and soybean trypsin inhibitor was loaded onto a column containing 6.56 ml Macro-Prep DEAE support. The sample was loaded in 50 mM Tris-HCl, pH 7.6 (buffer A) and eluted in a gradient of 0–1 M NaCl in buffer A as follows: buffer A for 15 ml, 0–0.35 M NaCl in 85 ml, 0.35–0.65 M NaCl in 15 ml, 0.65–1 M NaCl in 15 ml.
Macro-Prep High Q media: Effect of flow rate on peak symmetry and separation. A 5 ml sample (4.4 mg/ml) of myoglobin (peak 1), ovalbumin (peak 2), and BSA (peak 3) was run on a 1 x 13 cm (8.7 ml) column at each of the indicated flow rates. The buffer was 50 mM Tris-HCl, pH 8.6, with a gradient of 0–0.5 M NaCl over 100 ml.
Ammonium sulfate fraction of Hevea (rubber plant) cytosol on Macro-Prep 25 Q (11 x 21 cm). Buffer A, 50 mM Tris, pH 8.3; buffer B, 50 mM Tris, 1 M NaCl, pH 8.3; flow rate = 5 ml/min.
Separation on 360µl of 6x His-tagged dihydrofolate reductase E. coli lysate on a 2 ml column packed with Macro-Prep 25 Q Media. Buffer A: 50 mM Tris, pH 8.3. Buffer B: 50 mM Tris, pH 8.3, 1 M NaCl; gradient: 0–100% B in 10 column volumes; flow rate: 2 ml/min.
Sample separation with the Macro-Prep DEAE support and two other commercially available DEAE supports. A 100 µl sample containing 9 mg/ml each of myoglobin, conalbumin, ovalbumin, BSA, and soybean trypsin inhibitor was loaded onto a 0.5 cm ID column containing 2 ml of support. Sample was loaded onto the column in 50 mM Tris-HCl, pH 7.6 (buffer A) and was eluted with 1 M NaCl in buffer A. The flow rate was 153 cm/hr (0.5 ml/min). Scales offset for comparison.
Call us at 1-800-4-BIORAD (1-800-424-6723)
Please reenter your email address in the correct format.
Please enter your email address.
Your subscription information already exists, we will send you an email with specific instructions to manage your existing subscription profile.
To receive the latest news, promotions, and more, sign up for Bio-Rad updates by entering your email address below. You can elect to receive only the types of Bio-Rad communications that are of interest to you..
Copyright © 2016 Bio-Rad Laboratories, Inc. All rights reserved.