Lipid transfection reagents provide optimal transfection efficiencies for nucleic acid delivery into mammalian cells. General use and RNAi-optimized transfection reagents are available.
Bio-Rad offers lipid transfection reagents that effectively deliver nucleic acids to a wide range of cultured mammalian cell lines, including ones that are difficult to transfect. Each lipid is optimal for certain applications. The siLentFect™ lipid reagent is an RNAi-specific lipid that is ideal for siRNA delivery. It can also be used to perform cotransfection with a small reporter-plasmid along with the siRNA molecule. The TransFectin™ lipid reagent is a general-purpose lipid that is ideal for plasmid delivery in a wide range of cell lines.
* siLentFect lipid reagent can successfully cotransfect an siRNA molecule with a plasmid DNA. ** For a more complete list of cell lines, go to www.bio-rad.com/transfection.
The Transfection Protocol Online Library contains protocols obtained from the literature, developed by Bio-Rad scientists, or submitted by scientists like you. browse protocols to view our library and find your starting point.
Bio-Rad Laboratories has helped scientists deliver molecules into cells for over 35 years. Instruments, including the Gene Pulser® series of electroporators, the MicroPulser™ electroporator, and the Helios® gene gun and PDS-1000/He™ biolistic systems, have been used successfully to transfect prokaryotic and eukaryotic cells, including plant and animal cells. These transfection instruments are cited extensively in the literature. Our chemically mediated methods include lipid-based transfection reagents — TransFectin™ lipid reagent for delivery of plasmid DNA and siLentFect™ lipid reagent for siRNA delivery.
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