Digital PCR is a breakthrough technology that provides ultrasensitive and absolute nucleic acid quantification. It is particularly useful for low abundance targets, targets in complex backgrounds, allelic variants (SNPs), and for monitoring subtle changes in target levels that cannot be detected with real-time PCR.
Simply enter your target of interest or sequence and order a ddPCR Assay.
QX200™ AutoDG™ Droplet Digital™ PCR SystemFor high-throughput analysis
QX200™ Droplet Digital™ PCR (ddPCR™) SystemFor analyzing up to 96 samples
ddSEQ™ Single-Cell Isolator For single-cell gene expression analysis
ddPCR SupermixesFor sensitive DNA target detection
PrimePCR™ ddPCR™ AssaysExperimentally validated primer pairs
Digital PCR Library Quantification KitsFor quantifying sequencing libraries
ddPCR OilsFor creating sample-in-oil droplets
ddPCR ConsumablesFor use with the droplet generator
PX1™ Plate SealerFor secure plate sealing
Based on water-emulsion droplet technology, Droplet Digital PCR fractionates a DNA sample in 20,000 droplets. PCR amplification of the template subsequently occurs in each individual droplet, and counting the positive droplets gives precise, absolute target quantification.
Read more about Droplet Digital PCR technology.
DNA sample with target sequence is partitioned into droplets
Target and background DNA are randomly distributed among 20,000 droplets
Target sequence is amplified by end-point PCR in each droplet
Positive droplets are counted to give precise quantification of target sequences in sample
Absolute quantification — provides an absolute count of target DNA copies per input sample without the need for standard curves
Highest precision and sensitivity — enriched template concentration in target-positive droplets allows higher precision and sensitivity than real-time PCR
High throughput — simple and easy-to-use workflow with 96-sample throughput does not require multiple dilution steps
Removal of PCR efficiency bias — error rates are reduced by removing the reliance on amplification efficiency of qPCR
Copy number variation (CNV) Accurate CNV quantification in single wells
Next generation sequencingAmplification and accurate quantification of NGS libraries with validation of sequencing results
Detection of rare sequences in cancer and diseaseDetection of mutations and viral load below levels detectable by current tests
Gene expression analysisDetection of rare mRNAs and miRNAs with rapid turnover
Pathogen detection and microbiome analysisViral load and pathogen detection in organ transplants, soil, and food
Single cell analysisHigh sensitivity make it a robust tool for single-cell analyses
Download the Droplet Digital PCR Applications Guide for in-depth information about setting up experiments.
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