Bio-Rad offers four types of affinity chromatography media: Profinity, for affinity-tagged protein purification; Affi-Gel, for laboratory-scale affinity purification; Affi-Prep, for process-scale affinity purification;and UNOsphere SUPrA, for monoclonalantibody purification.
* Refer to bulletin 3193 for purification conditions. ** For example, Profinity GST resin is only available in prepacked cartridges. ***Profinity eXact purification resin.
Profinity epoxide resin is an activated affinity chromatography support for the immobilization of biomolecules that contain nucleophiles such as amino,thiol, or hydroxyl groups.
Profinity IMAC (immobilized metal affinity chromatography) resin is an affinity chromatography support for protein purification of recombinant histidine-tagged products.
Chromatography on Affi-Gel and Affi-Prep protein A media yields highly purified immunoglobulins (IgG), selectively removes IgG from serum, and adsorbs immune complexes for antigen affinity purification.
Affi-Gel Blue gel is a crosslinked agarose bead with covalently attached Cibacron Blue F3GA dye. The blue dye functions as an ionic, hydrophobic, aromatic, or sterically active binding site.
DEAE Affi-Gel Blue gel is a bifunctional matrix for both affinity purification and ion exchange chromatography prepared by coupling Cibacron Blue F3GA and diethylaminoethyl groups to Bio-Gel A.
CM Affi-Gel Blue gel contains Cibacron Blue F3GA dye covalently coupled to CM Bio-Gel A. This bifunctionalaffinity chromatography gel binds both albumin andserum proteases.
Affi-Gel heparin affinity chromatography gel is usefulin protein purification for a range of products such as coagulation factors, plasma proteins, polynucleotide polymerases, nucleases, lipases, lipoproteins,and proteases.
Affi-Prep polymyxin affinity chromatography supportconsists of 2–4 mg of USP-grade polymyxin per ml of the macroporous polymeric Affi-Prep support. It binds endotoxins from a number of strains ofgram-negative bacteria.
Affi-Gel boronate-derivatized polyacrylamide gel has ahigh binding capacity and is ideal for the affinity purificationof molecules with adjacent coplanar cis-hydroxylgroups (cis-diols).
Affi-Gel 10 and Affi-Gel 15 gels are activated affinity chromatography media that provide spontaneous, rapid, and highly efficient coupling of ligands via primary amines.
Affi-Gel Hz hydrazide gel activated affinitychromatography support couples immunoglobulin G (IgG) molecules via carbohydrate moieties on the Fc regionof antibody molecules.
Affi-Gel 102 affinity chromatography support is for use with 1-ethyl-3–(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC) coupling reagent, which immobilizes ligands that contain primary or terminal carboxyl groups.
UNOsphere SUPrA affinity chromatography media are designed for process-scale purification of monoclonal antibodies. These affinity media are built on the proven UNOsphere matrix for predictable performance.
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