Activated Affinity Media

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Overview

Activated media for spontaneous ligand immobilization allow for the creation of an affinity matrix of choice by coupling a ligand, such as an antibody, enzyme, or antigen.

Affinity Resin Selection Guide

Affinity Chromatography Resin Selection Guide

	Matrix	Functional Group	Specificity	Capacity	Working pH	Pressure Limit	Applications
Ready-to-Use Affinity Media
Nuvia™ IMAC	High capacity, pressure-stable polymer based on UNOsphere™ beads	NTA charged with Ni²⁺	Histidine	≥40 mg/ml*	2–14	45 psi (3.1 bar)	Purification of recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity™ IMAC	Pressure-stable polymer based on UNOsphere beads	IDA, available charged with Ni²⁺ and uncharged	Histidine	≥15 mg/ml*	1–14	100 psi (6.8 bar)	Suitable for purification of larger and difficult-to-purify recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity GST**	Pressure-stable polymer based on UNOsphere beads	Immobilized glutathione	GST-tagged proteins	≥10 mg/ml	1–14	45 psi (3.1 bar)	Purification of recombinant GST-tagged proteins
Profinity eXact™***	Crosslinked 6% agarose	Subtilisin protease	Subtilisin prodomain	≥3 mg/ml tag-free protein	2–10	10 psi (minus system pressure)	Generation of native, tag-free proteins by on-column purification and cleavage
Affi-Gel^® Protein A	Crosslinked agarose	Protein A 2 mg/ml	IgG		2–10	15 psi (1 bar)	Purification of IgG from ascites, serum, and culture fluid; with MAPS buffer system, purification of 10 mg mouse IgG per ml of gel is possible
Affi-Prep^® Protein A	Pressure-stable polymer	Protein A 2 mg/ml	IgG		2–10	1,000 psi (70 bar)	Purified IgG from ascites, serum, and culture fluid; pressure-stable support for process-scale applications
Affi-Gel Blue	Crosslinked agarose	Cibacron Blue F3GA 1.9 mg/ml	Albumin; general	≥11 mg/ml	2–10	15 psi (1 bar)	Binds many nucleotide-requiring enzymes, albumin, and other proteins
DEAE Affi-Gel Blue	Crosslinked agarose	Cibacron Blue F3GA and DEAE	Albumin and serum proteins	0.14 ml serum/ml gel	2–10	15 psi (1 bar)	Purifies protease-free IgG from ascites, serum, and culture fluid with minimal sample preparation
CM Affi-Gel Blue	Crosslinked agarose	Cibacron Blue F3GA and CM	Albumin and serum proteins	0.17–0.5 ml serum/ml gel	2–11	15 psi (1 bar)	Produces albumin- and protease-free antibody preparation from serum without prior dialysis
Affi-Prep Polymyxin	Pressure-stable polymer	Polymyxin 2–4 mg/ml	Endotoxins	>5 mg/ml	2–10	1,000 psi (70 bar)	Endotoxin removal
Affi-Gel Boronate	Polyacrylamide gel	Boronate 1.05 ± 0.15	cis-diols meq/g	130 µmol sorbitol/ml	2–10	15 psi (1 bar)	Adsorption of cis-hydroxyl–containing molecules, including sugars, nucleotides, and glycopeptides
Activated Media for Spontaneous Ligand Immobilization
Profinity Epoxide	Pressure-stable polymer based on UNOsphere beads	Epoxy group	Nucleophiles; amini, thiol, -COOH	36–40 mg/ml lgG	1–14	Up to 80 psi (5.5 bar)	Activated matrix for the immobilization of various ligands (for example, protein A, StrepTactin, and immunoglobulins)
Affi-Gel 10	Crosslinked agarose	N-hydroxy-succinimide ≥10 µmol/ml	-NH₂	35 mg/ml	3–11	15 psi (1 bar)	For coupling proteins with pI 6.5–11
Affi-Gel 15	Crosslinked agarose	N-hydroxy-succinimide ≥9 µmol/ml	-NH₂	35 mg/ml	3–11	15 psi (1 bar)	For coupling proteins with pI 6.5–11
Affi-Gel Hz	Crosslinked agarose	Hydrazide	Oxidized carbohydrates	1–5 mg/ml	2–10	15 psi (1 bar)	Immobilization of immunoglobulins and other glycoproteins via carbohydrate molecules
Affinity Media Using Carbodiimide Activation
Affi-Gel 102	Crosslinked agarose	-NH₂ 16 ± 4 meq/ml	-COOH	40 mg	2–11	15 psi (1 bar)	Carbodiimide coupling of carboxyl-containing ligand

* Refer to Bulletin 3193 for purification conditions.
** For example, Profinity GST Resin is available only in prepacked cartridges.
***Profinity eXact Purification Resin.

Affi-Gel 10 and 15 Activated Affinity Media

Category Products

Profinity Epoxide Resin
Profinity epoxide resin is an activated affinity chromatography support for the immobilization of ligands that contain nucleophiles, such as amino, thiol, or hydroxyl groups.

Affi-Gel 10 Gel
Affi-Gel 10 and Affi-Gel 15 activated affinity media provide spontaneous, rapid, and highly efficient coupling of ligands via primary amines.

Affi-Gel 15 Gel
Affi-Gel 10 and Affi-Gel 15 activated affinity media provide spontaneous, rapid, and highly efficient coupling of ligands via primary amines.

Affi-Gel Hz Hydrazide Gel
Affi-Gel Hz hydrazide gel activated support couples immunoglobulin G (IgG) molecules via carbohydrate moieties on the Fc region of antibody molecules.

Affi-Gel 102 Gel for Carbodiimide Activation
This support is for use with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC) carbodiimide coupling reagent, which immobilizes ligands that contain primary or terminal carboxyl groups.

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Activated Affinity Media

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