The Western Blot Doctor is a self-help guide that enables you to troubleshoot your western blotting problems. In this section, you can find solutions to issues related to loading controls.
Other sections in the Western Blot Doctor:
Click on the thumbnail that is most representative of your own blot to discover the probable causes and find specific solutions to the problem.
Protein level varies among samples
Protein detection saturated
Publications on total protein normalization as a loading control in western blotting — Bio-Rad bulletin 6349
Publications on the pitfalls of using housekeeping proteins for normalization in western blotting — Bio-Rad bulletin 6350
Publications on using stain-free technology for loading controls in western blotting — Bio-Rad bulletin 6351
Heidebrecht F et al. Improved semiquantitative western blot technique with increased quantification range. J Immunol Methods 345(1–2), 40–8 (2009).
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