Troubleshooting 2-D Electrophoresis Gels with 2-D Doctor™

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en-us LUSQQRB9O 2-D Doctor Troubleshooting 2-D Electrophoresis Gels with 2-D Doctor&trade; /webroot/web/html/lsr/solutions/technologies/2d_electrophoresis <p>The 2-D Doctor is a self-help guide developed by Bio-Rad that enables you to identify and troubleshoot your 2-D gel issues. Comprehensive solutions and suggestions are provided to help solve 2-D gel electrophoresis challenges.</p> <p><strong>Related Topics:</strong> <a href="/evportal/destination/solutions?catID=LUSQH6HYP">Protein Sample Preparation for 2-D Electrophoresis</a>, <a href="/evportal/destination/solutions?catID=LUSQLK2B7">First-Dimension Separation (Isoelectric Focusing)</a>, <a href="/evportal/destination/solutions?catID=LUSQMI97Q">Second-Dimension Separation</a>, <a href="/evportal/destination/solutions?catID=LUSQN83Q3">Staining and Visualization of Proteins After 2-D Electrophoresis</a>, <a href="/evportal/destination/solutions?catID=LUSQON470">Imaging and Analysis of 2-D Electrophoresis Gels</a> and <a href="/evportal/destination/solutions?catID=LUSQPTOZR">Protein Spot Excision and Protein Identification</a>.</p> <p>To start troubleshooting your 2-D gel problems choose the type of issue you are experiencing: horizontal streaking, vertical streaking or other.</p> <div class="clear">&nbsp;</div> <div class="contentroundwrap"> <div class="contentroundtop"> <h3>I am having trouble with:</h3> </div> <div class="contentroundmidwrap"> <div class="contentroundmid"> <table class="pd_table" border="0"> <tbody> <tr> <td><a href="/evportal/destination/solutions?catID=LUSQRG30E"><img src="/webroot/web/images/lsr/solutions/technologies/2-D_electrophoresis/2-D_electrophoresis/technology_detail/2dt17_img1.jpg" alt="Horizontal Streaking" width="165" height="161" border="0" /></a></td> <td><a href="/evportal/destination/solutions?catID=LUSQS649"><img src="/webroot/web/images/lsr/solutions/technologies/2-D_electrophoresis/2-D_electrophoresis/technology_detail/2dt17_img2.jpg" alt="Vertical Streaking" width="160" height="161" border="0" /></a></td> <td><a href="/evportal/destination/solutions?catID=LUSQTG84"><img src="/webroot/web/images/lsr/solutions/technologies/2-D_electrophoresis/2-D_electrophoresis/technology_detail/2dt17_img3.jpg" alt="Irregular Spot Patterns" width="160" height="161" border="0" /></a></td> </tr> </tbody> </table> </div> <div class="contentroundbot">&nbsp;</div> </div> </div> <br /> <script>// <![CDATA[ $(document).ready(function(){ $(".pagecontent2").hide(); }); // ]]></script> <!--- <p>&nbsp;</p> <div class="contentroundwrap"> <div class="contentroundtop"> <h3>Download the 2-D Doctor</h3> <a href="#helptop">Back to Top</a></div> <div class="contentroundmidwrap"> <div class="contentroundmid"> <p>For your convenience, you can download the 2-D Doctor for access when not connected to the Internet. Download 2-D Doctor by clicking the button below to choose your preferred language, and select Save File. The file will be in .zip format. After saving the zip file on your computer, extract the files in a folder that you specify. Once the files are extracted, double-click on the Start.html file to begin. The 2-D Doctor downloadable file will be updated regularly, so don't forget to check back for the most recent version.</p> <table class="pd_table" border="0"> <tbody> <tr> <td><a href="/pfamily/downloads/2D_doc_en_v2.zip"><img src="http://www.bio-rad.com/webroot/web/images/lsr/solutions/technologies/2-D_electrophoresis/2-D_electrophoresis/technology_detail/2dt17_english_btn.jpg" alt="English" width="129" height="32" border="0" /></a></td> <td><a href="/pfamily/downloads/2D_doc_ch_simp.zip"><img src="http://www.bio-rad.com/webroot/web/images/lsr/solutions/technologies/2-D_electrophoresis/2-D_electrophoresis/technology_detail//2dt17_chinese_btn.jpg" alt="Chinese" width="191" height="32" border="0" /></a></td> </tr> </tbody> </table> </div> <div class="contentroundbot">&nbsp;</div> </div>---> Protocols <table id="carttablealigned" class="literature_table" style="height: auto; width: 583px;" cellspacing="0" cellpadding="0" border="0"> <tbody> <tr> <th>Number</th> <th>Description</th> <th class="options">Options</th> </tr> <tr> <td>6222</td> <td>IPG Equilibration for the Second Dimension, Placement and Agarose Embedding of IPG Strips</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6222.pdf" target="_blank" rel="noopener noreferrer"><span>Click to download</span></a></td> </tr> <tr> <td>6236</td> <td>Using Precison Plus Protein Standard Plugs</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6236.pdf" target="_blank" rel="noopener noreferrer"><span>Click to download</span></a></td> </tr> </tbody> </table> <div class="videowrap vwrap_last"> <div class="videoImg"><a title="2-D Video Tutorial" onclick="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/electrophoresis/product_overlay/global/2-d_tutorial_video.html');" href="javascript:void(0);"> <img style="border: none;" src="/webroot/web/images/lsr/support/tutorials/global/2d_tutorial.jpg" alt="" /></a></div> <div class="videoDesc"><a title="2-D Video Tutorial" onclick="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/electrophoresis/product_overlay/global/2-d_tutorial_video.html');" href="javascript:void(0);">2-D Video Tutorial</a><br /> How to run a 2-D Gel from start to finish.</div> <div class="clear">&nbsp;</div> </div> 2651 2-D Electrophoresis Workflow How-To Guide, Rev F 2651 /webroot/web/pdf/lsr/literature/Bulletin_2651.pdf Literature PDF Manuals_and_Quick_Guides /webroot/web/images/general/icons/icon_pdf.gif 2-D Electrophoresis Workflow How-To Guide No 2-D Electrophoresis Workflow How-To Guide, Rev F 2651 proteomics, bulletin 2651, two dimensional electrophoresis, LIT2651, sample preparation, 2d, analytical, proteomeworks, prefractionation, 2-d manual, 2-d guide, 2-d electrophoresis workflow, 2-d methods and product, 2d manual, 2d guide, 2d electrophoresis workflow, 2d methods and product 2587 2587 High-Performance 2-D Gel Electrophoresis Using Narrow pH-Range ReadyStrip IPG Strips, Rev C /webroot/web/pdf/lsr/literature/Bulletin_2587.pdf Literature PDF Application Notes /webroot/web/images/general/icons/icon_pdf.gif No High-Performance 2-D Gel Electrophoresis Using Narrow pH-Range ReadyStrip IPG Strips, Rev C Life Science 2587 bulletin 2587, LIT2587, proteomeworks 2670 2670 Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen <i>Flavobacterium psychrophilum,</i> Using a 2-D Electrophoretic Approach /webroot/web/pdf/lsr/literature/Bulletin_2670.pdf Literature PDF Application Notes /webroot/web/images/general/icons/icon_pdf.gif No Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen <i>Flavobacterium psychrophilum,</i> Using a 2-D Electrophoretic Approach Life Science 2670 UNO Sphere, bulletin 2670, LIT2670 2740 Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A 2740 /webroot/web/pdf/lsr/literature/Bulletin_2740.pdf Literature PDF Application_Notes /webroot/web/images/general/icons/icon_pdf.gif No Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A 2740 LIT2740, bulletin 2740 2778 2778 Focusing Strategy and Influence of Conductivity on Isoelectric Focusing in Immobilized pH Gradients, Rev A /webroot/web/pdf/lsr/literature/Bulletin_2778.pdf Literature PDF Application Notes /webroot/web/images/general/icons/icon_pdf.gif No Focusing Strategy and Influence of Conductivity on Isoelectric Focusing in Immobilized pH Gradients, Rev A Life Science 2778 LIT2778, 2-d electrophoresis, ief 2859 2859 Combination of 2-D Gel and Liquid-Phase Electrophoretic Separations As Proteomic Tools in Neuroscience, Rev A /webroot/web/pdf/lsr/literature/Bulletin_2859.pdf Literature PDF Application Notes /webroot/web/images/general/icons/icon_pdf.gif No Combination of 2-D Gel and Liquid-Phase Electrophoretic Separations As Proteomic Tools in Neuroscience, Rev A Life Science 2859 sample preparation, LIT2859, prep cell, rotofor, bulletin 2859 3098 Expression Proteomics Overview Brochure, Rev B 3098 /webroot/web/pdf/lsr/literature/Bulletin_3098.pdf Literature PDF Brochures_and_Specifications /webroot/web/images/general/icons/icon_pdf.gif No Expression Proteomics Overview Brochure, Rev B 3098 2-D gel electrophoresis, 2D, mass spectrometry, membrane protein isolation, proteins, 2DGE, 3098, LIT3098, MALDI, ion-exchange chromatography, sample preparation, tools, 2-D electrophoresis, PDQuest image analysis software, bulletin 3098, imaging, 2 D, LC-MS/MS, preparative-scale, protein 5545 5545 Sensitivity and Protein-to-Protein Consistency of Flamingo Fluorescent Gel Stain Compared to Other Fluorescent Stains (Poster), Rev A /webroot/web/pdf/lsr/literature/5545RevA.pdf Literature PDF Scientific Posters /webroot/web/images/general/icons/icon_pdf.gif No Sensitivity and Protein-to-Protein Consistency of Flamingo Fluorescent Gel Stain Compared to Other Fluorescent Stains (Poster), Rev A Life Science 5545 LIT5545, 161-0492, 1610491, 1610492, 161-0490, 161-0491, 1610490 6097 PROTEAN i12 IEF System Brochure, Rev A 6097 H /webroot/web/pdf/lsr/literature/Bulletin_6097A.pdf Literature PDF Brochures_and_Specifications /webroot/web/images/general/icons/icon_pdf.gif No PROTEAN i12 IEF System Brochure, Rev A 6097 Bulletin 6097, 6138, 6139, 6140, 6142, two-dimensional gel electrophoresis, 2-D protein separation; 2DGE; isoelectric focusing (IEF); SDS-PAGE; broad, narrow and micro range ReadyStrip immobilized pH gradient (IPG) strips; sample rehydration method; guidelines for choice of IPG strips, rehydration method, IPG strip orientation, and loading method; focusing set up using gel side down, gel side up or gel side up with cup loading; creating, editing and programming run protocols; monitoring runs; saving, exporting and analyzing data and results; individual lane control; run multiple lanes with different samples, protocols and pH gradients; optimization in fewer runs; perform multiple experiments at once; 12 separately programmable power supplies; simultaneously optimize as many as 12 conditions; precise current limit delivered; touch-screen user interface; i12 Reporter; free web-based application; obtain reproducible data and extended separation over multiple pH ranges; narrow and micro pH ranges provide enhanced resolution; tight gel-length tolerances guarantee pH consistency; 164-6000, 1646000, 164-6001, 1646001, 163-2000, 1632000, 163-2001, 1632001, 163-2002, 1632002, 163-2003, 1632003, 163-2004, 1632004, 163-2005, 1632005, 163-2028, 1632028, 163-2029, 1632029, 163-2030, 1632030, 163-2031, 1632031, 163-2014, 1632014, 163-2015, 1632015, 163-2016, 1632016, 163-2017, 1632017, 163-2018, 1632018, 163-2019, 1632019, 163-2024, 1632024, 163-2025, 1632025, 163-2026, 1632026, 163-2027, 1632027, 163-2007, 1632007, 163-2008, 1632008, 163-2009, 1632009, 163-2010, 1632010, 163-2011, 1632011, 163-2012, 1632012, 163-2020, 1632020, 163-2021, 1632021, 163-2022, 1632022, 163-2023, 1632023, 163-2032, 1632032, 163-2033, 1632033, 163-2034, 1632034, 163-2035, 1632035, 163-2036, 1632036, 163-2037, 1632037, 163-2038, 1632038, 163-2039, 1632039, 163-2040, 1632040, 163-2041, 1632041, 163-2042, 1632042, 163-2043, 1632043, 163-2044, 1632044, 163-2045, 1632045, 163-2046, 1632046, 163-2047, 1632047, 163-2048, 1632048, 163-2049, 1632049, 163-2050, 1632050, 163-2051, 1632051, 163-2105, 1632105, 161-0378, 1610378 2621 PROTEAN Plus Dodeca Cell System Brochure, Rev C 2621 /webroot/web/pdf/lsr/literature/Bulletin_2621C.pdf Literature PDF Brochures_and_Specifications /webroot/web/images/general/icons/icon_pdf.gif No PROTEAN Plus Dodeca Cell Brochure, Rev C 2621 Bulletin 2621, second-dimension electrophoresis; 2DGE; two-dimensional (2-D) protein separation of complex mixtures of proteins; multi-casting chamber; Model 495 gradient former; AnyGel stand; Dodeca stainer; immobilized pH gradients (IPG) strips; uniform buffer temperature, electric field, high resolution via efficient cooling for large-format gels; 12-gel capacity; PROTEAN i12 IEF system; no buffer leaks; perfectly aligned plates eliminate potential current leaks; plate electrode give straight horizontal runs; easy assembly and cleanup; 165-4160, 1654160, 165-4120, 1654120, 165-4121, 1654121, 165-4122, 1654122, 165-4123, 1654123, 165-5131, 1655131, 165-4131, 1654131, 165-3400, 1653400, 165-3401, 1653401, 165-3403, 1653403, 165-3404, 1653404, 165-3405, 1653405, 165-3406, 1653406, 165-3407, 1653407, 165-3408, 1653408, 164-6000, 1646000, 164-6001, 1646001, 163-2000, 1632000, 163-2001, 1632001, 163-2002, 1632002, 163-2003, 1632003, 163-2004, 1632004, 163-2005, 1632005, 163-2028, 1632028, 163-2029, 1632029, 163-2030, 1632030, 163-2031, 1632031, 163-2014, 1632014, 163-2015, 1632015, 163-2016, 1632016, 163-2017, 1632017, 163-2018, 1632018, 163-2019, 1632019, 163-2024, 1632024, 163-2025, 1632025, 163-2026, 1632026, 163-2027, 1632027, 163-2007, 1632007, 163-2008, 1632008, 163-2009, 1632009, 163-2010, 1632010, 163-2011, 1632011, 163-2012, 1632012, 163-2020, 1632020, 163-2021, 1632021, 163-2022, 1632022, 163-2023, 1632023, 163-2032, 1632032, 163-2033, 1632033, 163-2034, 1632034, 163-2035, 1632035, 163-2036, 1632036, 163-2037, 1632037, 163-2038, 1632038, 163-2039, 1632039, 163-2040, 1632040, 163-2041, 1632041, 163-2042, 1632042, 163-2043, 1632043, 163-2044, 1632044, 163-2045, 1632045, 163-2046, 1632046, 163-2047, 1632047, 163-2048, 1632048, 163-2049, 1632049, 163-2050, 1632050, 163-2051, 1632051, 161-0378, 1610378 3095 2-D Electrophoresis: Tools for Rapid, High-Resolution Protein Separations Brochure, Rev B 3095 /webroot/web/pdf/lsr/literature/Bulletin_3095.pdf Literature PDF Brochures_and_Specifications /webroot/web/images/general/icons/icon_pdf.gif No 2-D Electrophoresis: Tools for Rapid, High-Resolution Protein Separations Brochure, Rev B 3095 bulletin 3095, dimension, gradient, multi casting, resolution, sds page, sulfate-, 2-D SDS-PAGE, 2D, first-dimension separation, proteins, ReadyStrip, separation, sodium dodecyl sulfate, SYPRO Ruby, Dodeca, first, IEF, MicroRotofor, PROTEAN II, expression proteomics, IPG strips, polyacrylamide gel electrophoresis, ReadyPrep, second, stainer, AnyGel stand, casting, Mini gels, Plus, precast, Ready Strip, sdspage, sulphate-, Blue R-250, LIT3095, Micro Rotofor, multi-casting, premixed buffer, Bio-Safe Coomassie, Criterion gel, Flamingo fluorescent, Mini-PROTEAN Tetra cell, multi-cell, Rotofor, silver, Model 491 Prep, Molecular Imager PharosFX, Ready Gel, second-dimension, stains, sulphate 3096 Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B 3096 /webroot/web/pdf/lsr/literature/Bulletin_3096.pdf Literature PDF Brochures_and_Specifications /webroot/web/images/general/icons/icon_pdf.gif Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure No Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B 3096 bio-spin, dynamic range reduction, sample prep, preparation, prep cell, rotofor, enrichment of low-abundance, isoelectric focusing, lit3096, membrane i, micro bio spin columns, model 491, proteins, truview cuvettes, cell lysis, expression proteomics, mini, prep, proteominer kits, quick start bradford assay, ready prep, readyprep, 2-d electrophoresis, affi-gel blue, albumin, aurum, chemical reagents, ief, microrotofor, protean ii, rc dc, smartspec plus, spectrophotometer, 165-2435, 1652435 3097 Imaging and Analysis: Tools for Acquisition and Analysis of Protein Expression Data Brochure, Rev B 3097 /webroot/web/pdf/lsr/literature/Bulletin_3097.pdf Literature PDF Brochures_and_Specifications /webroot/web/images/general/icons/icon_pdf.gif No Imaging and Analysis: Imaging and Analysis: Tools for Acquisition and Analysis of Protein Expression Data Brochure, Rev B 3097 1-D gels, gel, GS-800 Calibrated densitometer, Personal Molecular Imager system, pdquest, Pharos FX, VersaDoc, 5000, image, MP 4000, spot detection, ChemiDoc XRS, 2-d, Gel Doc XR, gels, protein, systems, 2d, PMI, proteins, 3097, bulletin 3097, PharosFX Plus, 2d electrophoresis, cutting, expression proteomics, EXQuest spot cutter, LIT3097, pdquest 2-d software, proteins staining, Quantity one software 268441258 268441565 268441566 268441989 268438765 268438804 268439702 1987 Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/2D-Electrophoresis/2-D Buffers and Reagents/ReadyPrep 2-D starter kit ->MT::6a7bf285-fd23-43c4-bdd8-c4f8012d4aef##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/2D-Electrophoresis/1st Dimension- Isoelectric Focusing (IEF)/ReadyStrip IPG Strips ->MT::56994cbe-ea1b-48d8-b7e9-bc17726c0d70##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/2D-Electrophoresis/1st Dimension- Isoelectric Focusing (IEF)/PROTEAN IEF Cell ->MT::92682b19-051c-464f-8a73-97cd256b225f##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Protein Standards/Recombinant Standards (Ladders)/Precision Plus Protein Standard Plugs ->MT::0c7564b9-7eec-48d8-b071-068a67e04ce4##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Midi Format 1D-Electrophoresis Systems/Criterion Cell Systems ->MT::b94e30f5-0f2a-452d-a3a0-825e8205b476## Life Science Research/Solutions/Applications/2-D Electrophoresis and Analysis ->MTS::LUSQF04EH## Life Science Research/Solutions/Technologies/Protein Electrophoresis ->MTS::LUSOVO47B##Life Science Research/Solutions/Technologies/Protein Electrophoresis/Protein Detection and Analysis/Protein Staining ->MTS::LUSPMPE8Z##Life Science Research/Solutions/Technologies/Imaging and Analysis ->MTS::LUSQC6MNI##Life Science Research/Solutions/Technologies/Western Blotting ->MTS::LUSPPAKG4## Karen Moss Troubleshooting 2D Electrophoresis Gels 12/23/11 11:40 AM 12/23/21 11:51 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Technologies/2-D_Electrophoresis N 0 Horizontal Streaking /en-us/applications-technologies/applications-technologies/horizontal-streaking?ID=LUSQRG30E Vertical Streaking /en-us/applications-technologies/applications-technologies/vertical-streaking?ID=LUSQS649 Irregular Spot Patterns /en-us/applications-technologies/applications-technologies/irregular-spot-patterns?ID=LUSQTG84 2-D Electrophoresis /en-us/applications-technologies/applications-technologies/troubleshooting-2-d-electrophoresis-gels-with-2-d-doctor?ID=LUSQG6LPT

The 2-D Doctor is a self-help guide developed by Bio-Rad that enables you to identify and troubleshoot your 2-D gel issues. Comprehensive solutions and suggestions are provided to help solve 2-D gel electrophoresis challenges.

Related Topics: Protein Sample Preparation for 2-D Electrophoresis, First-Dimension Separation (Isoelectric Focusing), Second-Dimension Separation, Staining and Visualization of Proteins After 2-D Electrophoresis, Imaging and Analysis of 2-D Electrophoresis Gels and Protein Spot Excision and Protein Identification.

To start troubleshooting your 2-D gel problems choose the type of issue you are experiencing: horizontal streaking, vertical streaking or other.

 

I am having trouble with:

Horizontal Streaking Vertical Streaking Irregular Spot Patterns
 

 
 
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    Literature
    Number Description Download
    2651 2-D Electrophoresis Workflow How-To Guide, Rev F Click to download
    2587 High-Performance 2-D Gel Electrophoresis Using Narrow pH-Range ReadyStrip IPG Strips, Rev C Click to download
    2670 Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen <i>Flavobacterium psychrophilum,</i> Using a 2-D Electrophoretic Approach Click to download
    2740 Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A Click to download
    2778 Focusing Strategy and Influence of Conductivity on Isoelectric Focusing in Immobilized pH Gradients, Rev A Click to download
    2859 Combination of 2-D Gel and Liquid-Phase Electrophoretic Separations As Proteomic Tools in Neuroscience, Rev A Click to download
    3098 Expression Proteomics Overview Brochure, Rev B Click to download
    5545 Sensitivity and Protein-to-Protein Consistency of Flamingo Fluorescent Gel Stain Compared to Other Fluorescent Stains (Poster), Rev A Click to download
    6097 PROTEAN i12 IEF System Brochure, Rev A Click to download
    2621 PROTEAN Plus Dodeca Cell System Brochure, Rev C Click to download
    3095 2-D Electrophoresis: Tools for Rapid, High-Resolution Protein Separations Brochure, Rev B Click to download
    3096 Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B Click to download
    3097 Imaging and Analysis: Tools for Acquisition and Analysis of Protein Expression Data Brochure, Rev B Click to download
    Number Description Options
    6222 IPG Equilibration for the Second Dimension, Placement and Agarose Embedding of IPG Strips Click to download
    6236 Using Precison Plus Protein Standard Plugs Click to download
     
     
    LUSQQRB9O [x-forwarded-proto] = [http] [x-forwarded-port] = [80] [x-forwarded-for] = [23.20.223.212, 10.232.18.83] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-us/applications-technologies/troubleshooting-2-d-electrophoresis-gels-with-2-d-doctor] [x-amzn-trace-id] = [Root=1-5b53b067-fa98a3eaf5720ac80e24d546] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=LUSQQRB9O] [host] = [10.232.16.28:1776] [x-request-uri] = [/en-us/applications-technologies/troubleshooting-2-d-electrophoresis-gels-with-2-d-doctor] [connection] = [Keep-Alive] [accept-encoding] = [x-gzip, gzip, deflate] [user-agent] = [CCBot/2.0 (http://commoncrawl.org/faq/)] AppTech/AppTechDetails pageStyleKey internet/solutions_sub applications-technologies/troubleshooting-2-d-electrophoresis-gels-with-2-d-doctor LSR LUSQQRB9O 2-D Doctor Troubleshooting 2-D Electrophoresis Gels with 2-D Doctor&trade; /webroot/web/html/lsr/solutions/technologies/2d_electrophoresis <p>The 2-D Doctor is a self-help guide developed by Bio-Rad that enables you to identify and troubleshoot your 2-D gel issues. Comprehensive solutions and suggestions are provided to help solve 2-D gel electrophoresis challenges.</p> <p><strong>Related Topics:</strong> <a href="/evportal/destination/solutions?catID=LUSQH6HYP">Protein Sample Preparation for 2-D Electrophoresis</a>, <a href="/evportal/destination/solutions?catID=LUSQLK2B7">First-Dimension Separation (Isoelectric Focusing)</a>, <a href="/evportal/destination/solutions?catID=LUSQMI97Q">Second-Dimension Separation</a>, <a href="/evportal/destination/solutions?catID=LUSQN83Q3">Staining and Visualization of Proteins After 2-D Electrophoresis</a>, <a href="/evportal/destination/solutions?catID=LUSQON470">Imaging and Analysis of 2-D Electrophoresis Gels</a> and <a href="/evportal/destination/solutions?catID=LUSQPTOZR">Protein Spot Excision and Protein Identification</a>.</p> <p>To start troubleshooting your 2-D gel problems choose the type of issue you are experiencing: horizontal streaking, vertical streaking or other.</p> <div class="clear">&nbsp;</div> <div class="contentroundwrap"> <div class="contentroundtop"> <h3>I am having trouble with:</h3> </div> <div class="contentroundmidwrap"> <div class="contentroundmid"> <table class="pd_table" border="0"> <tbody> <tr> <td><a href="/evportal/destination/solutions?catID=LUSQRG30E"><img src="/webroot/web/images/lsr/solutions/technologies/2-D_electrophoresis/2-D_electrophoresis/technology_detail/2dt17_img1.jpg" alt="Horizontal Streaking" width="165" height="161" border="0" /></a></td> <td><a href="/evportal/destination/solutions?catID=LUSQS649"><img src="/webroot/web/images/lsr/solutions/technologies/2-D_electrophoresis/2-D_electrophoresis/technology_detail/2dt17_img2.jpg" alt="Vertical Streaking" width="160" height="161" border="0" /></a></td> <td><a href="/evportal/destination/solutions?catID=LUSQTG84"><img src="/webroot/web/images/lsr/solutions/technologies/2-D_electrophoresis/2-D_electrophoresis/technology_detail/2dt17_img3.jpg" alt="Irregular Spot Patterns" width="160" height="161" border="0" /></a></td> </tr> </tbody> </table> </div> <div class="contentroundbot">&nbsp;</div> </div> </div> <br /> <script>// <![CDATA[ $(document).ready(function(){ $(".pagecontent2").hide(); }); // ]]></script> <!--- <p>&nbsp;</p> <div class="contentroundwrap"> <div class="contentroundtop"> <h3>Download the 2-D Doctor</h3> <a href="#helptop">Back to Top</a></div> <div class="contentroundmidwrap"> <div class="contentroundmid"> <p>For your convenience, you can download the 2-D Doctor for access when not connected to the Internet. Download 2-D Doctor by clicking the button below to choose your preferred language, and select Save File. The file will be in .zip format. After saving the zip file on your computer, extract the files in a folder that you specify. Once the files are extracted, double-click on the Start.html file to begin. The 2-D Doctor downloadable file will be updated regularly, so don't forget to check back for the most recent version.</p> <table class="pd_table" border="0"> <tbody> <tr> <td><a href="/pfamily/downloads/2D_doc_en_v2.zip"><img src="http://www.bio-rad.com/webroot/web/images/lsr/solutions/technologies/2-D_electrophoresis/2-D_electrophoresis/technology_detail/2dt17_english_btn.jpg" alt="English" width="129" height="32" border="0" /></a></td> <td><a href="/pfamily/downloads/2D_doc_ch_simp.zip"><img src="http://www.bio-rad.com/webroot/web/images/lsr/solutions/technologies/2-D_electrophoresis/2-D_electrophoresis/technology_detail//2dt17_chinese_btn.jpg" alt="Chinese" width="191" height="32" border="0" /></a></td> </tr> </tbody> </table> </div> <div class="contentroundbot">&nbsp;</div> </div>---> Protocols <table id="carttablealigned" class="literature_table" style="height: auto; width: 583px;" cellspacing="0" cellpadding="0" border="0"> <tbody> <tr> <th>Number</th> <th>Description</th> <th class="options">Options</th> </tr> <tr> <td>6222</td> <td>IPG Equilibration for the Second Dimension, Placement and Agarose Embedding of IPG Strips</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6222.pdf" target="_blank" rel="noopener noreferrer"><span>Click to download</span></a></td> </tr> <tr> <td>6236</td> <td>Using Precison Plus Protein Standard Plugs</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6236.pdf" target="_blank" rel="noopener noreferrer"><span>Click to download</span></a></td> </tr> </tbody> </table> <div class="videowrap vwrap_last"> <div class="videoImg"><a title="2-D Video Tutorial" onclick="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/electrophoresis/product_overlay/global/2-d_tutorial_video.html');" href="javascript:void(0);"> <img style="border: none;" src="/webroot/web/images/lsr/support/tutorials/global/2d_tutorial.jpg" alt="" /></a></div> <div class="videoDesc"><a title="2-D Video Tutorial" onclick="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/electrophoresis/product_overlay/global/2-d_tutorial_video.html');" href="javascript:void(0);">2-D Video Tutorial</a><br /> How to run a 2-D Gel from start to finish.</div> <div class="clear">&nbsp;</div> </div> 2651 2587 2670 2740 2778 2859 3098 5545 6097 2621 3095 3096 3097 268441258 268441565 268441566 268441989 268438765 268438804 268439702 1987 Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/2D-Electrophoresis/2-D Buffers and Reagents/ReadyPrep 2-D starter kit ->MT::6a7bf285-fd23-43c4-bdd8-c4f8012d4aef##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/2D-Electrophoresis/1st Dimension- Isoelectric Focusing (IEF)/ReadyStrip IPG Strips ->MT::56994cbe-ea1b-48d8-b7e9-bc17726c0d70##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/2D-Electrophoresis/1st Dimension- Isoelectric Focusing (IEF)/PROTEAN IEF Cell ->MT::92682b19-051c-464f-8a73-97cd256b225f##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Protein Standards/Recombinant Standards (Ladders)/Precision Plus Protein Standard Plugs ->MT::0c7564b9-7eec-48d8-b071-068a67e04ce4##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Midi Format 1D-Electrophoresis Systems/Criterion Cell Systems ->MT::b94e30f5-0f2a-452d-a3a0-825e8205b476## Life Science Research/Solutions/Applications/2-D Electrophoresis and Analysis ->MTS::LUSQF04EH## Life Science Research/Solutions/Technologies/Protein Electrophoresis ->MTS::LUSOVO47B##Life Science Research/Solutions/Technologies/Protein Electrophoresis/Protein Detection and Analysis/Protein Staining ->MTS::LUSPMPE8Z##Life Science Research/Solutions/Technologies/Imaging and Analysis ->MTS::LUSQC6MNI##Life Science Research/Solutions/Technologies/Western Blotting ->MTS::LUSPPAKG4## Karen Moss Troubleshooting 2D Electrophoresis Gels 12/23/11 11:40 AM 12/23/21 11:51 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Technologies/2-D_Electrophoresis N 0
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