Troubleshooting 2-D Electrophoresis Gels with 2-D Doctor™



The 2-D Doctor is a self-help guide developed by Bio-Rad that enables you to identify and troubleshoot your 2-D gel issues. Comprehensive solutions and suggestions are provided to help solve 2-D gel electrophoresis challenges.

Related Topics: Protein Sample Preparation for 2-D Electrophoresis, First-Dimension Separation (Isoelectric Focusing), Second-Dimension Separation, Staining and Visualization of Proteins After 2-D Electrophoresis, Imaging and Analysis of 2-D Electrophoresis Gels and Protein Spot Excision and Protein Identification.

To start troubleshooting your 2-D gel problems choose the type of issue you are experiencing: horizontal streaking, vertical streaking or other.


I am having trouble with:

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Horizontal Streaking Vertical Streaking Irregular Spot Patterns


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For your convenience, you can download the 2-D Doctor for access when not connected to the Internet. Download 2-D Doctor by clicking the button below to choose your preferred language, and select Save File. The file will be in .zip format. After saving the zip file on your computer, extract the files in a folder that you specify. Once the files are extracted, double-click on the Start.html file to begin. The 2-D Doctor downloadable file will be updated regularly, so don't forget to check back for the most recent version.

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2-D Video Tutorial
How to run a 2-D Gel from start to finish.
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2651 2-D Electrophoresis Workflow How-To Guide, Rev F Click to download
2587 High-Performance 2-D Gel Electrophoresis Using Narrow pH-Range ReadyStrip IPG Strips, Rev C Click to download
2670 Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach Click to download
2740 Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A Click to download
2778 Focusing Strategy and Influence of Conductivity on Isoelectric Focusing in Immobilized pH Gradients, Rev A Click to download
2859 Combination of 2-D Gel and Liquid-Phase Electrophoretic Separations As Proteomic Tools in Neuroscience, Rev A Click to download
3098 Expression Proteomics Overview Brochure, Rev B Click to download
5545 Sensitivity and Protein-to-Protein Consistency of Flamingo Fluorescent Gel Stain Compared to Other Fluorescent Stains (Poster), Rev A Click to download
6097 PROTEAN i12 IEF System Brochure, Rev A Click to download
2621 PROTEAN Plus Dodeca Cell System Brochure, Rev C Click to download
3095 2-D Electrophoresis: Tools for Rapid, High-Resolution Protein Separations Brochure, Rev B Click to download
3096 Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B Click to download
3097 Imaging and Analysis: Tools for Acquisition and Analysis of Protein Expression Data Brochure, Rev B Click to download
Number Description Options
6222 IPG Equilibration for the Second Dimension, Placement and Agarose Embedding of IPG Strips
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6236 Using Precison Plus Protein Standard Plugs
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