The 2-D Doctor is a self-help guide developed by Bio-Rad that enables you to identify and troubleshoot your 2-D gel issues. Comprehensive solutions and suggestions are provided to help solve 2-D gel electrophoresis challenges.
Related Topics: Protein Sample Preparation for 2-D Electrophoresis, First-Dimension Separation (Isoelectric Focusing), Second-Dimension Separation, Staining and Visualization of Proteins After 2-D Electrophoresis, Imaging and Analysis of 2-D Electrophoresis Gels and Protein Spot Excision and Protein Identification.
To start troubleshooting your 2-D gel problems choose the type of issue you are experiencing: horizontal streaking, vertical streaking or other.
For your convenience, you can download the 2-D Doctor for access when not connected to the Internet. Download 2-D Doctor by clicking the button below to choose your preferred language, and select Save File. The file will be in .zip format. After saving the zip file on your computer, extract the files in a folder that you specify. Once the files are extracted, double-click on the Start.html file to begin. The 2-D Doctor downloadable file will be updated regularly, so don't forget to check back for the most recent version.
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