Target Discovery: Identification and Validation




The first stage of drug development is target discovery, also known as target identification and validation, the process of identifying a potential target for a therapeutic drug for a particular disease or condition that does not have a treatment or for which existing therapeutic agents are lacking in some way. Thus, targets may be known, putative, or novel. Proteins are the most common targets and include enzymes, cell signaling receptors, structural proteins, and regulatory factors such as protein kinases. Other targets that are increasingly being investigated include nucleic acids such as mRNA, lipids, and carbohydrates. Show more

There are two main approaches to drug discovery, phenotype-based and target-based. Recently, there has been a resurgence in phenotype-based screening, which is monitoring changes cells in cells, tissues, or organisms. This type of screening has the ability to identify molecules and pathways that were previously not known to be involved in a disease. In target-based screening, each candidate target is validated by numerous experimental methods including modulation of the target to demonstrate that it is directly or indirectly involved in the disease or condition of interest.

In choosing a target, the probability of it being druggable must also be considered; that is, a drug must be able to bind the target and affect its function in a desirable way. Therefore, the target must be accessible to putative therapeutic agents, and the interaction should produce a measurable biological response. There are a wide range of experimental technologies used for validation, with the validation protocols varying depending on the function and characteristics of the target. Show less


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A Fast Semi-Automated Quantitative PCR Method for Monitoring Differential Gene Expression for Drug Target Discovery


Solutions for Target Discovery: Identification and Validation

Gene Expression

Gene Expression

Analyze gene expression, determine copy number variation (CNV), and initiate early biomarker discovery across a variety of biological samples with our range of validated PCR, qPCR, and Droplet Digital™ PCR instruments and reagents.

Droplet Digital PCR Systems
Real-Time PCR Automation
PCR Instrument Validation Tools and Services

Protein Expression

Protein Expression

Utilize multiplex immunoassays and our complete western blotting solutions, including gels, imagers, and validated antibodies, in your drug discovery process. Fast, reproducible assessment of proteins with built-in controls and validation steps for protein quantitation streamlines proteomics analysis and monitoring of protein expression levels.

Multiplex Cancer Immunoassays
Multiplex Human Inflammation Immunoassays
Western Blotting Reagents and Equipment
Stain-Free Gel and Western Blot Imaging Systems
Validated Western Blotting Antibodies
2-D Electrophoresis Systems

Cell Analysis

Cell Analysis

Explore our high-speed, high-purity automated cell sorter and easy-to-use automated cell counter; combine them with antibodies, reagents, and consumables for imaging and flow cytometry experiments to enable the execution of cell-based experiments such as target expression.

Cell Imaging
Cell Counting
Cell Sorting and Flow Cytometry
Antibodies for Flow Cytometry



Discover an extensive range of antibodies for applications such as flow cytometry, immunohistochemistry (IHC), western blotting, and ELISA to support your cell-based and proteomics target discovery. Custom services are available to meet your specific target identification and characterization needs

Antibodies by Application
Antibodies by Target Species
Cell Signaling Antibodies
HuCAL® Custom Antibody Services

Additional Methodologies and Research within Drug Discovery and Development

Biomarker Discovery

Biomarker Discovery
The use of biomarkers across drug discovery and development and into the clinic is key to enabling close monitoring of a drug candidate’s effects. Biomarkers can range from specific molecules such as a protein, a gene sequence, or expressed RNA to physiological parameters such as blood pressure. Biomarkers may be detected in a specific location or tissue or in plasma and other bodily fluids.

Biochemical and Cellular Assays

Biochemical and Cellular Assays
Biochemical and cellular pharmacology teams develop and validate assays to support research across drug discovery and development. These assays range from discovery approaches with high sample throughput to high-content analysis to gain an understanding of biological response both in vitro and in vivo.

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