Protein Electrophoresis Reagent Selection and Preparation

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en-us LUSP8GD57 Electrophoresis Reagent Selection and Preparation Protein Electrophoresis Reagent Selection and Preparation /webroot/web/html/lsr/solutions/technologies/protein_electrophoresis <p>This section details how to select and prepare the reagents (protein standards, gels, and buffers) required for various SDS-PAGE applications. The types of gels and buffers selected should suit the size of the protein under investigation, the desired analysis technique, and the overall goals of the experiment.</p> <p><strong>Related Topics:</strong> <a href="/evportal/destination/solutions?catID=LUSP9D7MU">Protein Standards</a>, <a href="/evportal/destination/solutions?catID=LUSPBRM5B">Polyacrylamide Gels</a>, <a href="/evportal/destination/solutions?catID=LUSPCXIDF">Buffer Systems and Gel Chemistries</a>,and <a href="/evportal/destination/solutions?catID=LUSPDTO62">Handcasting Polyacrylamide Gels</a>.</p> <h3 class="clear"><br /></h3> <h3 class="clear">General Considerations</h3> <p><img style="margin: 8px 0px 3px 0px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /></p> <p>No particular gel type or buffer is useful for all proteins, and choosing the buffer systems and gel types that offer the highest resolution in the size range of interest may require some experimentation. In selecting reagents for SDS-PAGE, consider the following:</p> <ul> <li><a href="/evportal/destination/solutions?catID=LUSP9D7MU#applications">Protein standards</a> &mdash; select protein standards that provide maximum resolution in the size range of interest and that offer compatibility and utility for downstream applications such as western blotting</li> <li><a href="/evportal/destination/solutions?catID=LUSPBRM5B#percentage">Gel percentage</a> &mdash; choose the percentage that offers resolution in the range of interest</li> <li><a href="/evportal/destination/solutions?catID=LUSPBRM5B#precast_v_handcast">Precast vs. handcast gels</a> &mdash; precast gels offer greater convenience and superior quality control and reproducibility than handcast gels; handcast gels offer the possibility of customized percentages and gradients</li> <li><a href="/evportal/destination/solutions?catID=LUSPBRM5B#format">Gel format</a> &mdash; select mini- or midi-format gels when throughput is important or sample size is limited; select large-format gels for higher resolution. Select a comb type and gel thickness to accommodate the sample number and volume you are working with</li> <li><a href="/evportal/destination/solutions?catID=LUSPCXIDF#gel_buffer_chemistries_for_page">Buffer system</a> &mdash; choose the system that offers the best resolution and compatibility with the protein and application of interest</li> </ul> 6040 /templatedata/internet/documentation/data/LSR/Literature/6040.xml Electrophoresis Guide, Interactive PDF, Rev C 6040 /webroot/web/pdf/lsr/literature/Bulletin_6040.pdf Literature PDF Manuals_and_Quick_Guides /webroot/web/images/general/icons/icon_pdf.gif No Electrophoresis Guide, Interactive PDF, Rev C 6040 Bulletin 2895, 6040, overview and principle, theory and product selection, one dimensional (1-D) protein separation, blue native polyacrylamide, SDS-PAGE, sodium dodecyl sulfate, sodium dodecyl sulphate, polymerization, precast, handcast resolving, stacking single-percentage gel, Laemmli (Tris-HCl), Bis-Tris, Tris-acetate, Tris-Tricine premade running and sample buffers, formulations/components, reagents, salts, isoelectric focusing (IEF), zymogram, gel casting chambers, gradient formers, sample preparation protocols, cell lysis, disruption, homogenization, protein solubilization, human suspension/monolayer cultured cells, mammalian tissue, plant leaves, microbial cultures, chromatography protein fractions, reducing, chaotropic agents, detergents, sample loading and quantitation spectrophotometric assays, recombinant and natural protein standards, prestained or unstained molecular weight markers or ladders, selecting power supplies, joule heating, separations under constant voltage, current, power, detection and analysis, total and specific protein staining, Coomassie Blue stain, fluorescent gel and silver stains, stainers, imagers, imaging systems and software, protein molecular weight (size) estimation, quantification, downstream applications, western blotting, gel drying, electroelution, spot excision (cutting), troubleshooting tips Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Protein Standards/Recombinant Standards (Ladders)/Precision Plus Protein Unstained Standards ->MT::ab429c02-ab15-4967-a7b9-1f78850fc4d3##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Protein Standards/Recombinant Standards (Ladders)/Precision Plus Protein Prestained Standards ->MT::a7b0f9ce-e080-4b51-ab99-4cded66497c1##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Mini Format 1D-Electrophoresis Systems/Mini-PROTEAN Precast Gels/Mini-PROTEAN TGX Precast Gels ->MTS::KSHTFUE8Z## Life Science Research/Solutions/Applications/Protein Separation and Analysis ->MTS::LUSOU3F7Q##Life Science Research/Solutions/Applications/2-D Electrophoresis and Analysis ->MTS::LUSQF04EH##Life Science Research/Solutions/Applications/Automated Electrophoresis and Analysis ->MTS::LUSQXB15##Life Science Research/Solutions/Technologies/Automated Electrophoresis System -Experion- ->MTS::LUSR04KG4## Eddie C Selection & Preparation of Electrophoresis Gels 12/16/11 09:21 AM 12/16/21 09:22 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Technologies/Protein_Electrophoresis N 0 Introduction to Western Blot Protein Standards /en-us/applications-technologies/applications-technologies/introduction-western-blot-protein-standards?ID=LUSP9D7MU Introduction to Polyacrylamide Gels /en-us/applications-technologies/applications-technologies/introduction-polyacrylamide-gels?ID=LUSPBRM5B Buffer Systems and Gel Chemistries /en-us/applications-technologies/applications-technologies/buffer-systems-gel-chemistries?ID=LUSPCXIDF Hand Casting Polyacrylamide Gels /en-us/applications-technologies/applications-technologies/hand-casting-polyacrylamide-gels?ID=LUSPDTO62 Introduction to Protein Electrophoresis /en-us/applications-technologies/applications-technologies/protein-electrophoresis-reagent-selection-preparation?ID=LUSOVO47B

This section details how to select and prepare the reagents (protein standards, gels, and buffers) required for various SDS-PAGE applications. The types of gels and buffers selected should suit the size of the protein under investigation, the desired analysis technique, and the overall goals of the experiment.

Related Topics: Protein Standards, Polyacrylamide Gels, Buffer Systems and Gel Chemistries,and Handcasting Polyacrylamide Gels.


General Considerations

No particular gel type or buffer is useful for all proteins, and choosing the buffer systems and gel types that offer the highest resolution in the size range of interest may require some experimentation. In selecting reagents for SDS-PAGE, consider the following:

  • Protein standards — select protein standards that provide maximum resolution in the size range of interest and that offer compatibility and utility for downstream applications such as western blotting
  • Gel percentage — choose the percentage that offers resolution in the range of interest
  • Precast vs. handcast gels — precast gels offer greater convenience and superior quality control and reproducibility than handcast gels; handcast gels offer the possibility of customized percentages and gradients
  • Gel format — select mini- or midi-format gels when throughput is important or sample size is limited; select large-format gels for higher resolution. Select a comb type and gel thickness to accommodate the sample number and volume you are working with
  • Buffer system — choose the system that offers the best resolution and compatibility with the protein and application of interest
 
 
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    6040 Electrophoresis Guide, Interactive PDF, Rev C Click to download
     
     
    LUSP8GD57 [x-forwarded-proto] = [http] [x-forwarded-port] = [80] [x-forwarded-for] = [66.249.79.33, 10.232.2.51] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [if-modified-since] = [Wed, 18 Jul 2018 00:28:24 GMT] [seourl] = [/en-us/applications-technologies/protein-electrophoresis-reagent-selection-preparation] [x-amzn-trace-id] = [Root=1-5b4f35ad-e25644e2ed320d38e82e7fa6] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=LUSP8GD57] [host] = [10.232.1.21:1776] [x-request-uri] = [/en-us/applications-technologies/protein-electrophoresis-reagent-selection-preparation] [from] = [googlebot(at)googlebot.com] [connection] = [Keep-Alive] [accept-encoding] = [gzip,deflate,br] [user-agent] = [Mozilla/5.0 (compatible; Googlebot/2.1; +http://www.google.com/bot.html)] AppTech/AppTechDetails pageStyleKey internet/solutions_sub applications-technologies/protein-electrophoresis-reagent-selection-preparation LSR LUSP8GD57 Electrophoresis Reagent Selection and Preparation Protein Electrophoresis Reagent Selection and Preparation /webroot/web/html/lsr/solutions/technologies/protein_electrophoresis <p>This section details how to select and prepare the reagents (protein standards, gels, and buffers) required for various SDS-PAGE applications. The types of gels and buffers selected should suit the size of the protein under investigation, the desired analysis technique, and the overall goals of the experiment.</p> <p><strong>Related Topics:</strong> <a href="/evportal/destination/solutions?catID=LUSP9D7MU">Protein Standards</a>, <a href="/evportal/destination/solutions?catID=LUSPBRM5B">Polyacrylamide Gels</a>, <a href="/evportal/destination/solutions?catID=LUSPCXIDF">Buffer Systems and Gel Chemistries</a>,and <a href="/evportal/destination/solutions?catID=LUSPDTO62">Handcasting Polyacrylamide Gels</a>.</p> <h3 class="clear"><br /></h3> <h3 class="clear">General Considerations</h3> <p><img style="margin: 8px 0px 3px 0px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /></p> <p>No particular gel type or buffer is useful for all proteins, and choosing the buffer systems and gel types that offer the highest resolution in the size range of interest may require some experimentation. In selecting reagents for SDS-PAGE, consider the following:</p> <ul> <li><a href="/evportal/destination/solutions?catID=LUSP9D7MU#applications">Protein standards</a> &mdash; select protein standards that provide maximum resolution in the size range of interest and that offer compatibility and utility for downstream applications such as western blotting</li> <li><a href="/evportal/destination/solutions?catID=LUSPBRM5B#percentage">Gel percentage</a> &mdash; choose the percentage that offers resolution in the range of interest</li> <li><a href="/evportal/destination/solutions?catID=LUSPBRM5B#precast_v_handcast">Precast vs. handcast gels</a> &mdash; precast gels offer greater convenience and superior quality control and reproducibility than handcast gels; handcast gels offer the possibility of customized percentages and gradients</li> <li><a href="/evportal/destination/solutions?catID=LUSPBRM5B#format">Gel format</a> &mdash; select mini- or midi-format gels when throughput is important or sample size is limited; select large-format gels for higher resolution. Select a comb type and gel thickness to accommodate the sample number and volume you are working with</li> <li><a href="/evportal/destination/solutions?catID=LUSPCXIDF#gel_buffer_chemistries_for_page">Buffer system</a> &mdash; choose the system that offers the best resolution and compatibility with the protein and application of interest</li> </ul> 6040 /templatedata/internet/documentation/data/LSR/Literature/6040.xml Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Protein Standards/Recombinant Standards (Ladders)/Precision Plus Protein Unstained Standards ->MT::ab429c02-ab15-4967-a7b9-1f78850fc4d3##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Protein Standards/Recombinant Standards (Ladders)/Precision Plus Protein Prestained Standards ->MT::a7b0f9ce-e080-4b51-ab99-4cded66497c1##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Mini Format 1D-Electrophoresis Systems/Mini-PROTEAN Precast Gels/Mini-PROTEAN TGX Precast Gels ->MTS::KSHTFUE8Z## Life Science Research/Solutions/Applications/Protein Separation and Analysis ->MTS::LUSOU3F7Q##Life Science Research/Solutions/Applications/2-D Electrophoresis and Analysis ->MTS::LUSQF04EH##Life Science Research/Solutions/Applications/Automated Electrophoresis and Analysis ->MTS::LUSQXB15##Life Science Research/Solutions/Technologies/Automated Electrophoresis System -Experion- ->MTS::LUSR04KG4## Eddie C Selection & Preparation of Electrophoresis Gels 12/16/11 09:21 AM 12/16/21 09:22 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Technologies/Protein_Electrophoresis N 0
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