This section details how to select and prepare the reagents (protein standards, gels, and buffers) required for various SDS-PAGE applications. The types of gels and buffers selected should suit the size of the protein under investigation, the desired analysis technique, and the overall goals of the experiment.
Related Topics: Protein Standards, Polyacrylamide Gels, Buffer Systems and Gel Chemistries,and Handcasting Polyacrylamide Gels.
No particular gel type or buffer is useful for all proteins, and choosing the buffer systems and gel types that offer the highest resolution in the size range of interest may require some experimentation. In selecting reagents for SDS-PAGE, consider the following:
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