Protein Electrophoresis Equipment

Print

Overview

en-us LUSOZFBEB Electrophoresis Equipment -Cells and Power Supplies- Protein Electrophoresis Equipment /webroot/web/html/lsr/solutions/technologies/protein_electrophoresis <p>Electrophoresis equipment includes cells and power supplies. This section gives a selection of cells and power supplies available from Bio-Rad and provides guidelines for their usage</p> <p><strong>Related Topics:</strong> <a href="/evportal/destination/solutions?catID=LUSOW4GRI">Protein Electrophoresis Methods</a>, <a href="/evportal/destination/solutions?catID=LUSP8GD57">Protein Electrophoresis Reagent Selection and Preparation</a> and <a href="/evportal/destination/solutions?catID=LUSPFBNEL">Performing Protein Electrophoresis</a>.</p> <p><a name="electrophoresis_cells"></a></p> Electrophoresis Cells <p><img src="/webroot/web/images/lsr/solutions/technologies/protein_electrophoresis_blotting_and_imaging/protein_electrophoresis/technology_detail/pet12_img1.jpg" alt="Components of a vertical electrophoresis cell" /></p> <p class="caption"><strong>Components of a vertical electrophoresis cell.</strong></p> <p>Vertical electrophoresis cells are manufactured in different size formats to accommodate different gels sizes. Cell selection is dependent on the requirements for speed, resolution, and throughput (both number of samples and number of gels) as well as the volume of sample available (see Table below).</p> <p><strong>Vertical Electrophoresis System Selection Guide</strong></p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td class="pd_tableheader pd_centeredcell" colspan="2"><strong><a href="http://www.bio-rad.com/en-us/product/electrophoresis-chambers/mini-protean-tetra-cell">Mini-PROTEAN<sup>&reg;</sup> System</a></strong></td> <td class="pd_tableheader pd_centeredcell"><strong><a href="http://www.bio-rad.com/en-us/product/electrophoresis-chambers/criterion-cell">Criterion&trade; System</a></strong></td> <td class="pd_tableheader pd_centeredcell"><strong><a href="http://www.bio-rad.com/en-us/product/large-format-1-d-electrophoresis-chambers/protean-ii-xi-cell">PROTEAN<sup>&reg;</sup> II System</a></strong></td> <td class="pd_tableheader pd_centeredcell"><strong><a href="http://www.bio-rad.com/en-us/product/large-format-1-d-electrophoresis-chambers/protean-plus-dodeca-cell">PROTEAN<sup>&reg;</sup> Plus System</a></strong></td> </tr> <tr> <td class="pd_centeredcell" colspan="2"><img src="/webroot/web/images/lsr/products/electrophoresis/category_overlay_content/global/precast_img_01.jpg" border="0" alt="" width="150" /></td> <td class="pd_centeredcell"><img src="/webroot/web/images/lsr/products/electrophoresis/category_overlay_content/global/precast_img_02.jpg" border="0" alt="" width="135" /></td> <td class="pd_centeredcell"><img src="/webroot/web/images/lsr/products/electrophoresis/category_overlay_content/global/precast_img_03.jpg" border="0" alt="" width="145" /></td> <td class="pd_centeredcell"><img src="/webroot/web/images/lsr/products/electrophoresis/category_thumb/global/protean-plus-dodeca-cell-thumb-large-format-1-D-electrophoresis-chambers.jpg" alt="" width="153" height="98" /></td> </tr> <tr class="pd_colorbackground" valign="top"> <td colspan="5"><strong>Advantages:</strong></td> </tr> <tr class="pd_colorbackground" valign="top"> <td colspan="2">Run 1&ndash;4 precast or handcast gels in the Mini-PROTEAN<sup>&reg;</sup> Tetra cell up to 12 gels in the Mini-PROTEAN<sup>&reg;</sup> Dodeca cell in mini format<br /><br /> Wing clamp assembly allows faster setup and leak-free operation <p>&nbsp;</p> </td> <td>Fast setup with drop-in gel and cell design (precast or handcast)<br /><br /> Run 1&ndash;2 Criterion&trade; precast or handcast gels<br /><br /> Integrated upper buffer chamber allows leak-free operation</td> <td>Large format gel system offers greater resolution over smaller formats and can be used with handcast or precast gels<br /><br /> Can accommodate up to 4 gels and is available in xi or XL format for running a variety of gel sizes<br /><br /> Multi-cell is available for running up to 6 gels</td> <td>Offers maximum resolution in a single gel and the longest range of separation (with the ability to run up to 12 gels)<br /><br /> Specifically for the second dimension of 2-d electrophoresis<br /><br /></td> </tr> <tr valign="top"> <td colspan="5"><strong>Compatible Gel Formats</strong></td> </tr> <tr valign="top"> <td>Precast</td> <td><a href="http://www.bio-rad.com/en-us/category/protein-gels/mini-protean-precast-gels">Mini-PROTEAN Precast Gels</a><br /><br /> <a href="http://www.bio-rad.com/en-us/category/protein-gels/ready-gel-precast-gels">Ready Gel<sup>&reg;</sup> Precast Gels</a></td> <td><a href="http://www.bio-rad.com/en-us/category/protein-gels/criterion-precast-gels">Criterion Precast Gels</a></td> <td>&nbsp;</td> <td>&nbsp;</td> </tr> <tr valign="top"> <td>Handcast</td> <td><a href="/en-us/product/electrophoresis-chambers/mini-protean-tetra-handcast-systems?tab=Plates">Mini-PROTEAN Empty Cassettes and Casting Plates</a><br /></td> <td><a href="/en-us/product/electrophoresis-chambers/criterion-cell?tab=Accessories">Criterion Empty Cassettes</a></td> <td><a href="/en-us/product/large-format-1-d-electrophoresis-chambers/protean-ii-xi-cell?tab=Accessories">PROTEAN II Casting Plates</a></td> <td><a href="/en-us/product/large-format-1-d-electrophoresis-chambers/protean-plus-hinged-spacer-plates-combs">PROTEAN Plus casting equipment</a></td> </tr> <tr class="pd_colorbackground" valign="top"> <td colspan="5"><strong>Electrophoresis Cells</strong></td> </tr> <tr class="pd_colorbackground" valign="top"> <td colspan="2"><a href="http://www.bio-rad.com/en-us/product/electrophoresis-chambers/mini-protean-tetra-cell">Mini-PROTEAN Tetra Cell</a>&nbsp; <br /><br /><a href="http://www.bio-rad.com/en-us/product/electrophoresis-chambers/mini-protean-3-dodeca-cell">Mini-PROTEAN<sup>&reg;</sup> 3 Dodeca</a><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=f9e3876d-3d8b-4e4c-965d-bc8a17dfc3d5">&trade; Cell<br /></a></td> <td><a href="http://www.bio-rad.com/en-us/product/electrophoresis-chambers/criterion-cell">Criterion Cell</a><br /><br /> <p><a href="http://www.bio-rad.com/en-us/product/electrophoresis-chambers/criterion-dodeca-cell">Criterion&trade; Dodeca</a><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=f9e3876d-3d8b-4e4c-965d-bc8a17dfc3d5">&trade;</a><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=f9e3876d-3d8b-4e4c-965d-bc8a17dfc3d5"> Cell<br /></a></p> </td> <td><a href="http://www.bio-rad.com/en-us/product/large-format-1-d-electrophoresis-chambers/protean-ii-xi-cell">PROTEAN II xi/XL Cell</a><br /><br /> <a href="http://www.bio-rad.com/en-us/product/large-format-1-d-electrophoresis-chambers/protean-ii-xi-xl-multi-cells">PROTEAN II xi/XL Multi-Cells</a></td> <td><a href="http://www.bio-rad.com/en-us/product/large-format-1-d-electrophoresis-chambers/protean-plus-dodeca-cell">PROTEAN Plus Dodeca</a></td> </tr> <tr> <td colspan="5"><strong>Precast Gel Dimensions</strong></td> </tr> <tr valign="top"> <td>(W x L x<br /> thickness)</td> <td>Mini-PROTEAN Precast Gels: <br /> 8.6 x 6.7 x 0.1 cm<br /><br /> Ready Gel Precast Gels: <br /> 8.3 x 6.4 x 0.1 cm</td> <td>Criterion Precast Gels:<br /> 13.3 x 8.7 x 0.1 cm</td> <td>&nbsp;</td> <td>&nbsp;</td> </tr> <tr class="pd_colorbackground" valign="top"> <td colspan="5"><strong>Cassette Dimensions (for Handcasting Gels)</strong></td> </tr> <tr class="pd_colorbackground" valign="top"> <td colspan="2">10.0 x 8.0 cm</td> <td>15.0 x 10.6 cm</td> <td>20.0 x 18.3 cm</td> <td>18.5 x 20.5 cm<br /> 20.0 x 20.5 cm <br /> 20.0 x 20.5 cm <br /></td> </tr> <tr valign="top"> <td colspan="5"><strong>Compatible Transfer Systems</strong></td> </tr> <tr valign="top"> <td>Wet/tank<br /> transfer</td> <td><a href="http://www.bio-rad.com/en-us/product/mini-trans-blot-cell">Mini Trans-Blot<sup>&reg;</sup> Cell</a> <br /><br /> <a href="http://www.bio-rad.com/en-us/product/criterion-blotter">Criterion Blotter </a><br /><br /> <a href="http://www.bio-rad.com/en-us/product/trans-blot-cell">Trans-Blot Cell</a></td> <td><a href="/en-us/product/criterion-blotter">Criterion Blotter</a><br /><br /> <a href="http://www.bio-rad.com/en-us/product/trans-blot-cell">Trans-Blot Cell </a><br /><br /> <a href="http://www.bio-rad.com/en-us/product/trans-blot-plus-cell">Trans-Blot Plus Cell</a></td> <td><a href="http://www.bio-rad.com/en-us/product/trans-blot-cell">Trans-Blot Cell </a><br /><br /> <a href="http://www.bio-rad.com/en-us/product/trans-blot-plus-cell">Trans-Blot Plus Cell</a></td> <td><a href="http://www.bio-rad.com/en-us/product/trans-blot-plus-cell">Trans-Blot Plus Cell </a></td> </tr> <tr valign="top"> <td>Semi-dry<br /> transfer</td> <td><a href="http://www.bio-rad.com/en-us/product/trans-blot-sd-semi-dry-transfer-cell">Trans-Blot SD Cell </a><br /><br /> <a href="/en-us/product/semi-dry-rapid-blotting-systems/trans-blot-turbo-transfer-system">Trans-Blot<sup>&reg;</sup> Turbo&trade; Transfer System</a></td> <td><a href="http://www.bio-rad.com/en-us/product/trans-blot-sd-semi-dry-transfer-cell">Trans-Blot SD Cell</a><br /><br /> <a href="/en-us/product/semi-dry-rapid-blotting-systems/trans-blot-turbo-transfer-system">Trans-Blot<sup>&reg;</sup> Turbo&trade; Transfer System</a></td> <td><a href="http://www.bio-rad.com/en-us/product/trans-blot-sd-semi-dry-transfer-cell">Trans-Blot SD Cell</a></td> <td>&nbsp;</td> </tr> </tbody> </table> <p><strong>Vertical Electrophoresis System Selection Guide</strong></p> <ul> <li><strong> Mini-format systems</strong> &mdash; accommodate small gels (up to 8.6 x 6.7 cm). The short separation distance maximizes the electrical field strength (V/cm) to yield rapid separations with moderate resolution. Use these systems for rapid analysis, method development, or when sample volumes are limited. The Mini-PROTEAN<sup>&reg;</sup> system includes the <a href="http://www.bio-rad.com/en-us/product/electrophoresis-chambers/mini-protean-tetra-cell">Mini-PROTEAN Tetra cell</a> (with a capacity of up to four gels) and the high-throughput <a href="http://www.bio-rad.com/en-us/product/electrophoresis-chambers/mini-protean-3-dodeca-cell">Mini-PROTEAN 3 Dodeca cell</a> (for running up to 12 gels)</li> <li><strong>Midi-format systems</strong> &mdash; accommodate 13.3 x 8.7 cm gels and offer rapid runs with higher sample capacity and enhanced separation over mini-format gels. The Criterion&trade; system includes the <a href="/en-us/product/electrophoresis-chambers/criterion-cell">Criterion Cell</a> (for 1&ndash;2 gels) and high-throughput <a href="http://www.bio-rad.com/en-us/product/electrophoresis-chambers/criterion-dodeca-cell">Criterion Dodeca cell</a> (for 1&ndash;12 gels); both cells are compatible with Criterion precast gels</li> <li><strong>Large-format systems</strong> &mdash; accommodate large gels (up to 20.0 x 18.3 cm for the PROTEAN<sup>&reg;</sup> II system, designed for one-dimensional PAGE) and so offer maximum resolution. The PROTEAN II system provides a choice of glass plates, spacer, and sandwich clamps to cast two gel lengths/ 10 or 20 cm. The PROTEAN<sup>&reg;</sup> Plus Dodeca&trade; cell allows maximum throughput with the capability to run up to 12 2-D gels at a time </li> </ul> <div class="top"><a href="#helptop">Back to Top</a></div> <p><a id="power_supplies_for_PAGE_apps" name="power_supplies_for_PAGE_apps"></a></p> Power Supplies for PAGE Applications <p>Power supplies are available to meet the power requirements of numerous applications (see below). The choice of power supply for PAGE applications usually depends on the size and number of gels being run. The following Table compares the Bio-Rad PowerPac&trade; power supplies recommended for vertical electrophoresis applications.</p> <ul> <li>For mini-format vertical PAGE applications, the <a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=bea5dea1-cef0-43ad-8af5-b2c0287f6e07">PowerPac Basic</a> and <a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=65e1d658-c899-4011-a7eb-406797c3c9e8">PowerPac HC</a> power supply are both suitable</li> <li>For large-format vertical PAGE applications, the <a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=b64403c7-43a2-4085-aba0-ce78c2b6f330">PowerPac HV</a> and <a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=89cb0329-d728-4689-87e8-47d77fd0a9f0">PowerPac Universal</a> power supply are both suitable</li> <li>For applications that require high currents, such as PAGE with the high-throughput Dodeca cells, the PowerPac HC power supply is the appropriate model</li> </ul> <p><strong>PowerPac power supplies selection guide</strong></p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td rowspan="2"><strong>Technique and Recommended Apparatus</strong></td> <td colspan="2"><strong>Typical Conditions* (Initial)</strong></td> <td colspan="2"><strong>Typical Conditions* (Final)</strong></td> <td rowspan="2"><strong>Run Time</strong></td> <td rowspan="2"><strong>PowerPac&trade; Power Supply</strong></td> </tr> <tr> <td><strong>V</strong></td> <td><strong>mA</strong></td> <td><strong>V</strong></td> <td><strong>mA</strong></td> </tr> <tr class="pd_colorbackground"> <td colspan="10"><strong>Laemmli (SDS), O'Farrell Second Dimension (SDS)</strong></td> </tr> <tr class="pd_colorbackground"> <td>PROTEAN<sup>&reg;</sup> II xi cell</td> <td>100</td> <td>35(C)</td> <td>350</td> <td>35(C)</td> <td>5 hr</td> <td>HV or Universal</td> </tr> <tr class="pd_colorbackground"> <td>PROTEAN II XL cell</td> <td>100</td> <td>35(C)</td> <td>350</td> <td>35(C)</td> <td>5 hr</td> <td>HV or Universal</td> </tr> <tr class="pd_colorbackground"> <td>Criterion&trade; cell</td> <td>200(C)</td> <td>200</td> <td>200(C)</td> <td>80</td> <td>55 min</td> <td>Basic or HC</td> </tr> <tr class="pd_colorbackground"> <td>Mini-PROTEAN<sup>&reg;</sup> Tetra cell</td> <td>200(C)</td> <td>240</td> <td>200(C)</td> <td>120</td> <td>35&ndash;45 min</td> <td>Basic or HC</td> </tr> <tr> <td colspan="10"><strong>High-Throughput Electrophoresis</strong></td> </tr> <tr> <td>PROTEAN Plus</td> <td>200(C)</td> <td>1,000</td> <td>200(C)</td> <td>350</td> <td>6 hr</td> <td>HC or Universal</td> </tr> <tr> <td rowspan="2">Dodeca&trade; cell</td> <td>200(C)</td> <td>1,000</td> <td>200(C)</td> <td>350</td> <td>6 hr</td> <td>HC or Universal</td> </tr> <tr> <td>150(C)</td> <td>1,200</td> <td>150(C)</td> <td>300</td> <td>18&ndash;20 hr</td> <td>HC or Universal</td> </tr> <tr> <td>PROTEAN II xi/XL multi-cell</td> <td>150</td> <td>480(C)</td> <td>500</td> <td>480(C)</td> <td>5 hr</td> <td>Universal</td> </tr> <tr> <td>Criterion&trade; Dodeca&trade; cell</td> <td>200(C)</td> <td>1,000&ndash;1,400</td> <td>200(C)</td> <td>400&ndash;500</td> <td>55 min</td> <td>HC or Universal</td> </tr> <tr> <td>Mini-PROTEAN<sup>&reg;</sup> 3 Dodeca&trade; cell</td> <td>200(C)</td> <td>600</td> <td>200(C)</td> <td>360</td> <td>45 min</td> <td>HC or Universal</td> </tr> <tr class="pd_colorbackground"> <td colspan="10"><strong>Western Blotting</strong></td> </tr> <tr class="pd_colorbackground"> <td>Mini Trans-Blot<sup>&reg;</sup> cell</td> <td>100(C)</td> <td>250</td> <td>100(C)</td> <td>450</td> <td>1 hr</td> <td>HC</td> </tr> <tr class="pd_colorbackground"> <td colspan="10">Criterion&trade; blotter</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;Wire electrodes</td> <td>100(C)</td> <td>250</td> <td>100(C)</td> <td>450</td> <td>1 hr</td> <td>HC</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;Plate electrodes</td> <td>100(C)</td> <td>650</td> <td>100(C)</td> <td>1,600</td> <td>30 min</td> <td>HC</td> </tr> <tr class="pd_colorbackground"> <td colspan="10">Trans-Blot<sup>&reg;</sup> cell</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;Wire electrodes</td> <td>60(C)</td> <td>210&ndash;250</td> <td>60(C)</td> <td>210&mdash;250</td> <td>5 hr</td> <td>HC</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;Plate electrodes</td> <td>100&ndash;150(C)</td> <td>1,000&ndash;1,600</td> <td>100&ndash;150(C)</td> <td>1,000&ndash;1,600</td> <td>1&ndash;5 hr</td> <td>HC</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;High-intensity transfer</td> <td>50&ndash;100(C)</td> <td>1,600</td> <td>50&ndash;100(C)</td> <td>1,600</td> <td>30 min</td> <td>HC</td> </tr> <tr class="pd_colorbackground"> <td>Trans-Blot<sup>&reg;</sup> Plus cell</td> <td>100(C)</td> <td>3,000</td> <td>100(C)</td> <td>3,000</td> <td>30 min&ndash;1 hr</td> <td>HC</td> </tr> <tr class="pd_colorbackground"> <td colspan="10">Trans-Blot<sup>&reg;</sup> SD cell</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;Protein</td> <td>15(C)</td> <td>500</td> <td>15(C)</td> <td>200</td> <td>15&ndash;30 min</td> <td>HC</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;DNA/RNA</td> <td>15</td> <td>650(C)</td> <td>25</td> <td>650(C)</td> <td>10&ndash;30 min</td> <td>HC</td> </tr> </tbody> </table> <p class="caption">*(C) = constant. Typical conditions are listed as guidelines only and can vary based on sample, buffers, etc.</p> <p><img src="/webroot/web/images/lsr/solutions/technologies/protein_electrophoresis_blotting_and_imaging/protein_electrophoresis/technology_detail/pet12_img2.jpg" alt="PowerPac power supplies" /></p> <p class="caption"><strong>PowerPac power supplies.</strong></p> <div class="top"><a href="#helptop">Back to Top</a></div> References <p>Andrews AT (1986). Electrophoresis: Theory, Techniques and Biochemical and Clinical Applications (New York: Oxford University Press).</p> <div class="top"><a href="#helptop">Back to Top</a></div> <div class="videowrap vwrap_last"> <div class="videoImg"><a title="Mini-PROTEAN Tetra Cell: Features, Parts, and Assembly" onclick="window.open('http://bio-rad.centra.com/bio-radroot/learning%20objects/mini-protean_tetra/mini-protean_tetra_cell.htm', 'StatusBar', 'toolbar=no, resizable=no, width=800, height=600');" href="javascript:void(0);"><img style="border:none;" src="https://www.bio-rad.com/webroot/web/images/lsr/support/tutorials/global/mini-protean.png" alt="" /></a></div> <div class="videoDesc"><a title="Mini-PROTEAN Tetra Cell: Features, Parts, and Assembly" onclick="window.open('http://bio-rad.centra.com/bio-radroot/learning%20objects/mini-protean_tetra/mini-protean_tetra_cell.htm', 'StatusBar', 'toolbar=no, resizable=no, width=800, height=600');" href="javascript:void(0);">Mini-PROTEAN<sup>&reg;</sup> Tetra Cell: Features, Parts, and Assembly</a><br /> This tutorial highlights the main features and components of the Mini-PROTEAN Tetra cell and shows you how to assemble the unit and its handcasting accessories. Information about the compatibility of its components with the Mini-PROTEAN 3 and Mini Trans-Blot<sup>&reg;</sup> cells is also provided.</div> <div class="clear">&nbsp;</div> </div> <div class="videowrap vwrap_last"> <div class="videoImg"><a title="Bio-Rad's PowerPac Family of Power Supplies" onclick="window.open('/webroot/web/html/lsr/support/lsr_electro_powerpac_supply.html', 'StatusBar', 'toolbar=no, resizable=no, width=800, height=600');" href="javascript:void(0);"><img style="border:none;" src="https://www.bio-rad.com/webroot/web/images/lsr/support/tutorials/global/thb_powerpacs.png" alt="" /></a></div> <div class="videoDesc"><a title="Bio-Rad's PowerPac Family of Power Supplies" onclick="window.open('/webroot/web/html/lsr/support/lsr_electro_powerpac_supply.html', 'StatusBar', 'toolbar=no, resizable=no, width=800, height=600');" href="javascript:void(0);">Bio-Rad's PowerPac&trade; Family of Power Supplies</a><br /> This tutorial describes the features of Bio-Rad's PowerPac family of power supplies and compares their specifications. It also offers information about parameter selection, common error messages, and available PowerPac accessories. Important safety information is also provided.</div> <div class="clear">&nbsp;</div> </div> 6040 /templatedata/internet/documentation/data/LSR/Literature/6040.xml Electrophoresis Guide, Interactive PDF, Rev C 6040 /webroot/web/pdf/lsr/literature/Bulletin_6040.pdf Literature PDF Manuals_and_Quick_Guides /webroot/web/images/general/icons/icon_pdf.gif No Electrophoresis Guide, Interactive PDF, Rev C 6040 Bulletin 2895, 6040, overview and principle, theory and product selection, one dimensional (1-D) protein separation, blue native polyacrylamide, SDS-PAGE, sodium dodecyl sulfate, sodium dodecyl sulphate, polymerization, precast, handcast resolving, stacking single-percentage gel, Laemmli (Tris-HCl), Bis-Tris, Tris-acetate, Tris-Tricine premade running and sample buffers, formulations/components, reagents, salts, isoelectric focusing (IEF), zymogram, gel casting chambers, gradient formers, sample preparation protocols, cell lysis, disruption, homogenization, protein solubilization, human suspension/monolayer cultured cells, mammalian tissue, plant leaves, microbial cultures, chromatography protein fractions, reducing, chaotropic agents, detergents, sample loading and quantitation spectrophotometric assays, recombinant and natural protein standards, prestained or unstained molecular weight markers or ladders, selecting power supplies, joule heating, separations under constant voltage, current, power, detection and analysis, total and specific protein staining, Coomassie Blue stain, fluorescent gel and silver stains, stainers, imagers, imaging systems and software, protein molecular weight (size) estimation, quantification, downstream applications, western blotting, gel drying, electroelution, spot excision (cutting), troubleshooting tips 5535 Mini-PROTEAN<sup>&reg;</sup> Electrophoresis System Brochure, Rev F 5535 H /webroot/web/pdf/lsr/literature/Bulletin_5535.pdf Literature PDF Brochures_and_Specifications /webroot/web/images/general/icons/icon_pdf.gif Mini-PROTEAN Electrophoresis System Brochure No Mini-PROTEAN<sup>&reg;</sup> Electrophoresis System Brochure, Rev F 5535 5535, mini-protean, miniprotean, mini protean, electrophoresis, tetra cell, tetracell, 3 dodeca, tgx stain-free, stain free, tris-tricine, tris tricine, tbe urea, tbe-urea, tris-hcl, tris hcl, ief, isoelectric focusing, zymogram, ready gel, mini trans-blot, trans blot turbo, transblot, sds-page, sds page, sodium dodecyl sulfate polyacrylamide, precast gel, hand-cast, hand cast, protein separation, 165-8000, 165-8001, 165-8002, 165-8003, 165-8004, 165-8005, 165-4100, 165-4101, 165-5132, 1658000, 1658001, 1658002, 1658003, 1658004, 1658005, 1654100, 1654101, 1655132 2710 Criterion™ Precast Gel System Brochure, Rev C 2710 /webroot/web/pdf/lsr/literature/Bulletin_2710.pdf Literature PDF Brochures_and_Specifications /webroot/web/images/general/icons/icon_pdf.gif No Criterion Precast Gel System Brochure, Rev C 2710 criterion, bulletin 2710, lit2710, 345-0123, 345-0124, 345-0125, 345-0126, 345-0111, 345-0112, 345-0113, 345-0117, 345-0118, 345-0119, 345-0120, 345-0127, 345-0115, 345-0121, 345-0129, 345-0130, 345-0131, 345-0135, 345-0136, 345-0133, 345-0009, 345-0010, 345-0011, 345-0012, 345-9949, 345-9950, 345-9951, 345-0045, 345-0042, 345-0043, 345-0044, 345-0014, 345-0015, 345-0016, 345-0017, 345-0019, 345-0020, 345-0021, 345-0022, 345-0023, 345-0024,, 345-0071, 345-0072, 345-0073, 345-0076, 3450123, 3450124, 3450125, 3450126, 3450111, 3450112, 3450113, 3450117, 3450118, 3450119, 3450120, 3450127, 3450115, 3450121, 3450129, 3450130, 3450131, 3450135, 3450136, 3450133, 3450009, 3450010, 3450011, 3450012, 3459949, 3459950, 3459951, 3450045, 3450042, 3450043, 3450044, 3450014, 3450015, 3450016, 3450017, 3450019, 3450020, 3450021, 3450022, 3450023, 3450024, 3450071, 3450072, 3450073, 3450076 1760 PROTEAN<sup>&reg;</sup> II xi and XL Cells PIS, Rev F 1760 H /webroot/web/pdf/lsr/literature/Bulletin_1760.pdf Literature PDF Product_Information_Sheets /webroot/web/images/general/icons/icon_pdf.gif No PROTEAN II xi and XL Cells PIS, Rev F 1760 bulletin 1760, large format electrophoresis, multi-cells, multi cells, ipg strips, page, handcast, readygel precast gels, tube gel, slab, preparative, gradient, sds-page, native, 1-d, 2-d, 1 d, 2, 165-1801, 165-1802, 165-1803, 165-1804, 165-1811, 165-1813, 165-1814, 165-3188, 165-3189, 165-3190, 165-3183, 165-3186, 165-3184, 165-1834, 165-1951, 165-1956, 165-3176, 165-3177, 165-3178, 165-1931, 165-1932, 165-1933, 165-1934, 165-1835, 165-3176JA, 165-3177JA, 165-3182, 165-3188JA, 165-3188RGC, 165-3189JA, 165-3190JA, 176-2710, 176-2720, 1651801, 1651802, 1651803, 1651804, 1651811, 1651813, 1651814, 1653188, 1653189, 1653190, 1653183, 1653186, 1653184, 1651834, 1651951, 1651956, 1653176, 1653177, 1653178, 1651931, 1651932, 1651933, 1651934, 1651835, 1653176JA, 1653177JA, 1653182, 1653188JA, 1653188RGC, 1653189JA, 1653190JA, 1762710, 1762720 2881 PowerPac Basic 300 V Power Supply Flier, Rev B 2881 /webroot/web/pdf/lsr/literature/Bulletin_2881B.pdf Literature PDF Brochures_and_Specifications /webroot/web/images/general/icons/icon_pdf.gif No PowerPac Basic 300 V Power Supply Flier, Rev B 2881 bulletin 2881, LIT2881, PowerPack, Pac, Pack 2882 PowerPac HC High-Current Power Supply Flier, Rev A 2882 /webroot/web/pdf/lsr/literature/Bulletin_2882A.pdf Literature PDF Brochures_and_Specifications /webroot/web/images/general/icons/icon_pdf.gif No PowerPac HC High-Current Power Supply Flier, Rev A 2882 164-5052, 165-4140, 170-3848, 170-3836, 170-3874, power pac, PROTEAN Dodeca, Trans-Blot Plus cell, 165-5051, 165-4138, 170-3991, Bulletin 2882, two-dimensional, 165-4141, 170-3853, SDS-PAGE, 170-3872, Criterion blotter, Trans Blot, 165-5066, 170-3825, 170-3850, high-throughput electrophoresis systems, SD 2885 PowerPac Universal Power Supply Brochure, Rev B 2885 /webroot/web/pdf/lsr/literature/Bulletin_2885.pdf Literature PDF Brochures_and_Specifications /webroot/web/images/general/icons/icon_pdf.gif No PowerPac Universal Power Supply Brochure, Rev B 2885 lit2885, bulletin 2885, power supply, power pack, powerpac, power pac, electrophoresis, 164-5070, 164-5070EDU, 164-5070J20, 164-5070J22, 164-5070J23, 164-5070J25, 164-5070J26, 164-5070J41, 164-5070J42, 164-5070J44, 164-5070J47, 164-5070J70, 164-5070J71, 164-5070J72, 164-5070J74, 164-5070J75, 164-5070J77, 164-5070J78, 164-5070J79, 164-5070JA, 165-4139, 165-4142, 165-4144, 165-4145, 165-8026, 170-3849, 170-3992, 1645070, 1645070EDU, 1645070J20, 1645070J22, 1645070J23, 1645070J25, 1645070J26, 1645070J41, 1645070J42, 1645070J44, 1645070J47, 1645070J70, 1645070J71, 1645070J72, 1645070J74, 1645070J75, 1645070J77, 1645070J78, 1645070J79, 1645070JA, 1654139, 1654142, 1654144, 1654145, 1658026, 1703849, 1703992 3189 PowerPac&trade; HV Power Supply Brochure 3189 /webroot/web/pdf/lsr/literature/Bulletin_3189.pdf Literature PDF Brochures_and_Specifications /webroot/web/images/general/icons/icon_pdf.gif PowerPac&trade; HV Power Supply Brochure No PowerPac&trade; HV Power Supply Brochure 3189 164-5059, 1645098, power pack, powerpack, powerpac, power supply, high-voltage, 164-5058, 164-5099, 1645099, lit3189, 164-5097, 1645059, 1645097, 1645058, 164-5098, 164-5059, 164-5056, 164-5056EDU, 164-5056JA, 164-5059EDU, 165-5048, 165-5048EDU, 165-8028, 170-2906, 170-2914, A30-10010, A30-10020, 1645056, 1645056EDU, 1645056JA, 1645059EDU, 1655048, 1655048EDU, 1658028, 1702906, 1702914, A3010010, A3010020 Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/2D-Electrophoresis/2nd Dimension Mini Format Electrophoresis Systems/Mini-PROTEAN Tetra Cell Systems ->MT::91d3ccf0-0657-4e53-be59-7b5446f6fb54##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Midi Format 1D-Electrophoresis Systems/Criterion Cell Systems ->MT::b94e30f5-0f2a-452d-a3a0-825e8205b476##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/2D-Electrophoresis/2nd Dimension Large Format Electrophoresis Systems/PROTEAN II xi Cells ->MT::15fb8e8f-b179-41d3-9222-6b96de3a0648##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/2D-Electrophoresis/1st Dimension- Isoelectric Focusing (IEF)/PROTEAN IEF Cell ->MT::92682b19-051c-464f-8a73-97cd256b225f##Life Science Research/Products/Electrophoresis and Blotting/Experion Automated Electrophoresis System/Experion Automated Electrophoresis Station ->MT::e88b061b-37dc-4610-b4b7-0ef925f622f9## Life Science Research/Solutions/Technologies/2-D Electrophoresis ->MTS::LUSQG6LPT##Life Science Research/Solutions/Technologies/Western Blotting ->MTS::LUSPPAKG4## Life Science Research/Solutions/Applications/Protein Separation and Analysis ->MTS::LUSOU3F7Q##Life Science Research/Solutions/Applications/2-D Electrophoresis and Analysis ->MTS::LUSQF04EH##Life Science Research/Solutions/Applications/Automated Electrophoresis and Analysis ->MTS::LUSQXB15##Life Science Research/Solutions/Technologies/Automated Electrophoresis System -Experion- ->MTS::LUSR04KG4## Eddie C Protein Electrophoresis Equipment 12/14/11 03:30 PM 12/14/21 03:32 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Technologies/Protein_Electrophoresis N 0 Introduction to Protein Electrophoresis /en-us/applications-technologies/applications-technologies/protein-electrophoresis-equipment?ID=LUSOVO47B

Electrophoresis equipment includes cells and power supplies. This section gives a selection of cells and power supplies available from Bio-Rad and provides guidelines for their usage

Related Topics: Protein Electrophoresis Methods, Protein Electrophoresis Reagent Selection and Preparation and Performing Protein Electrophoresis.

 

Electrophoresis Cells

Components of a vertical electrophoresis cell

Components of a vertical electrophoresis cell.

Vertical electrophoresis cells are manufactured in different size formats to accommodate different gels sizes. Cell selection is dependent on the requirements for speed, resolution, and throughput (both number of samples and number of gels) as well as the volume of sample available (see Table below).

Vertical Electrophoresis System Selection Guide

Mini-PROTEAN® System Criterion™ System PROTEAN® II System PROTEAN® Plus System
Advantages:
Run 1–4 precast or handcast gels in the Mini-PROTEAN® Tetra cell up to 12 gels in the Mini-PROTEAN® Dodeca cell in mini format

Wing clamp assembly allows faster setup and leak-free operation

 

Fast setup with drop-in gel and cell design (precast or handcast)

Run 1–2 Criterion™ precast or handcast gels

Integrated upper buffer chamber allows leak-free operation
Large format gel system offers greater resolution over smaller formats and can be used with handcast or precast gels

Can accommodate up to 4 gels and is available in xi or XL format for running a variety of gel sizes

Multi-cell is available for running up to 6 gels
Offers maximum resolution in a single gel and the longest range of separation (with the ability to run up to 12 gels)

Specifically for the second dimension of 2-d electrophoresis

Compatible Gel Formats
Precast Mini-PROTEAN Precast Gels

Ready Gel® Precast Gels
Criterion Precast Gels    
Handcast Mini-PROTEAN Empty Cassettes and Casting Plates
Criterion Empty Cassettes PROTEAN II Casting Plates PROTEAN Plus casting equipment
Electrophoresis Cells
Mini-PROTEAN Tetra Cell 

Mini-PROTEAN® 3 Dodeca™ Cell
Criterion Cell

Criterion™ Dodeca Cell

PROTEAN II xi/XL Cell

PROTEAN II xi/XL Multi-Cells
PROTEAN Plus Dodeca
Precast Gel Dimensions
(W x L x
thickness)
Mini-PROTEAN Precast Gels:
8.6 x 6.7 x 0.1 cm

Ready Gel Precast Gels:
8.3 x 6.4 x 0.1 cm
Criterion Precast Gels:
13.3 x 8.7 x 0.1 cm
   
Cassette Dimensions (for Handcasting Gels)
10.0 x 8.0 cm 15.0 x 10.6 cm 20.0 x 18.3 cm 18.5 x 20.5 cm
20.0 x 20.5 cm
20.0 x 20.5 cm
Compatible Transfer Systems
Wet/tank
transfer
Mini Trans-Blot® Cell

Criterion Blotter

Trans-Blot Cell
Criterion Blotter

Trans-Blot Cell

Trans-Blot Plus Cell
Trans-Blot Cell

Trans-Blot Plus Cell
Trans-Blot Plus Cell
Semi-dry
transfer
Trans-Blot SD Cell

Trans-Blot® Turbo™ Transfer System
Trans-Blot SD Cell

Trans-Blot® Turbo™ Transfer System
Trans-Blot SD Cell  

Vertical Electrophoresis System Selection Guide

  • Mini-format systems — accommodate small gels (up to 8.6 x 6.7 cm). The short separation distance maximizes the electrical field strength (V/cm) to yield rapid separations with moderate resolution. Use these systems for rapid analysis, method development, or when sample volumes are limited. The Mini-PROTEAN® system includes the Mini-PROTEAN Tetra cell (with a capacity of up to four gels) and the high-throughput Mini-PROTEAN 3 Dodeca cell (for running up to 12 gels)
  • Midi-format systems — accommodate 13.3 x 8.7 cm gels and offer rapid runs with higher sample capacity and enhanced separation over mini-format gels. The Criterion™ system includes the Criterion Cell (for 1–2 gels) and high-throughput Criterion Dodeca cell (for 1–12 gels); both cells are compatible with Criterion precast gels
  • Large-format systems — accommodate large gels (up to 20.0 x 18.3 cm for the PROTEAN® II system, designed for one-dimensional PAGE) and so offer maximum resolution. The PROTEAN II system provides a choice of glass plates, spacer, and sandwich clamps to cast two gel lengths/ 10 or 20 cm. The PROTEAN® Plus Dodeca™ cell allows maximum throughput with the capability to run up to 12 2-D gels at a time

 

Power Supplies for PAGE Applications

Power supplies are available to meet the power requirements of numerous applications (see below). The choice of power supply for PAGE applications usually depends on the size and number of gels being run. The following Table compares the Bio-Rad PowerPac™ power supplies recommended for vertical electrophoresis applications.

  • For mini-format vertical PAGE applications, the PowerPac Basic and PowerPac HC power supply are both suitable
  • For large-format vertical PAGE applications, the PowerPac HV and PowerPac Universal power supply are both suitable
  • For applications that require high currents, such as PAGE with the high-throughput Dodeca cells, the PowerPac HC power supply is the appropriate model

PowerPac power supplies selection guide

Technique and Recommended Apparatus Typical Conditions* (Initial) Typical Conditions* (Final) Run Time PowerPac™ Power Supply
V mA V mA
Laemmli (SDS), O'Farrell Second Dimension (SDS)
PROTEAN® II xi cell 100 35(C) 350 35(C) 5 hr HV or Universal
PROTEAN II XL cell 100 35(C) 350 35(C) 5 hr HV or Universal
Criterion™ cell 200(C) 200 200(C) 80 55 min Basic or HC
Mini-PROTEAN® Tetra cell 200(C) 240 200(C) 120 35–45 min Basic or HC
High-Throughput Electrophoresis
PROTEAN Plus 200(C) 1,000 200(C) 350 6 hr HC or Universal
Dodeca™ cell 200(C) 1,000 200(C) 350 6 hr HC or Universal
150(C) 1,200 150(C) 300 18–20 hr HC or Universal
PROTEAN II xi/XL multi-cell 150 480(C) 500 480(C) 5 hr Universal
Criterion™ Dodeca™ cell 200(C) 1,000–1,400 200(C) 400–500 55 min HC or Universal
Mini-PROTEAN® 3 Dodeca™ cell 200(C) 600 200(C) 360 45 min HC or Universal
Western Blotting
Mini Trans-Blot® cell 100(C) 250 100(C) 450 1 hr HC
Criterion™ blotter
  Wire electrodes 100(C) 250 100(C) 450 1 hr HC
  Plate electrodes 100(C) 650 100(C) 1,600 30 min HC
Trans-Blot® cell
  Wire electrodes 60(C) 210–250 60(C) 210—250 5 hr HC
  Plate electrodes 100–150(C) 1,000–1,600 100–150(C) 1,000–1,600 1–5 hr HC
  High-intensity transfer 50–100(C) 1,600 50–100(C) 1,600 30 min HC
Trans-Blot® Plus cell 100(C) 3,000 100(C) 3,000 30 min–1 hr HC
Trans-Blot® SD cell
  Protein 15(C) 500 15(C) 200 15–30 min HC
  DNA/RNA 15 650(C) 25 650(C) 10–30 min HC

*(C) = constant. Typical conditions are listed as guidelines only and can vary based on sample, buffers, etc.

PowerPac power supplies

PowerPac power supplies.

 

References

Andrews AT (1986). Electrophoresis: Theory, Techniques and Biochemical and Clinical Applications (New York: Oxford University Press).

 

Related Content

 
Mini-PROTEAN® Tetra Cell: Features, Parts, and Assembly
This tutorial highlights the main features and components of the Mini-PROTEAN Tetra cell and shows you how to assemble the unit and its handcasting accessories. Information about the compatibility of its components with the Mini-PROTEAN 3 and Mini Trans-Blot® cells is also provided.
 
Bio-Rad's PowerPac™ Family of Power Supplies
This tutorial describes the features of Bio-Rad's PowerPac family of power supplies and compares their specifications. It also offers information about parameter selection, common error messages, and available PowerPac accessories. Important safety information is also provided.
 
Literature
Number Description Download
6040 Electrophoresis Guide, Interactive PDF, Rev C Click to download
5535 Mini-PROTEAN<sup>&reg;</sup> Electrophoresis System Brochure, Rev F Click to download
2710 Criterion™ Precast Gel System Brochure, Rev C Click to download
1760 PROTEAN<sup>&reg;</sup> II xi and XL Cells PIS, Rev F Click to download
2881 PowerPac Basic 300 V Power Supply Flier, Rev B Click to download
2882 PowerPac HC High-Current Power Supply Flier, Rev A Click to download
2885 PowerPac Universal Power Supply Brochure, Rev B Click to download
3189 PowerPac&trade; HV Power Supply Brochure Click to download
 
 
LUSOZFBEB [x-forwarded-proto] = [http] [x-forwarded-port] = [80] [x-forwarded-for] = [66.249.75.25, 10.232.2.107] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [if-modified-since] = [Wed, 15 Aug 2018 23:58:18 GMT] [seourl] = [/en-us/applications-technologies/protein-electrophoresis-equipment] [x-amzn-trace-id] = [Root=1-5b75611f-961e8b69ba4f5df882e161a0] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=LUSOZFBEB] [host] = [10.232.16.28:1776] [x-request-uri] = [/en-us/applications-technologies/protein-electrophoresis-equipment] [from] = [googlebot(at)googlebot.com] [connection] = [Keep-Alive] [accept-encoding] = [gzip,deflate,br] [user-agent] = [Mozilla/5.0 (compatible; Googlebot/2.1; +http://www.google.com/bot.html)] AppTech/AppTechDetails pageStyleKey internet/solutions_sub applications-technologies/protein-electrophoresis-equipment LSR LUSOZFBEB Electrophoresis Equipment -Cells and Power Supplies- Protein Electrophoresis Equipment /webroot/web/html/lsr/solutions/technologies/protein_electrophoresis <p>Electrophoresis equipment includes cells and power supplies. This section gives a selection of cells and power supplies available from Bio-Rad and provides guidelines for their usage</p> <p><strong>Related Topics:</strong> <a href="/evportal/destination/solutions?catID=LUSOW4GRI">Protein Electrophoresis Methods</a>, <a href="/evportal/destination/solutions?catID=LUSP8GD57">Protein Electrophoresis Reagent Selection and Preparation</a> and <a href="/evportal/destination/solutions?catID=LUSPFBNEL">Performing Protein Electrophoresis</a>.</p> <p><a name="electrophoresis_cells"></a></p> Electrophoresis Cells <p><img src="/webroot/web/images/lsr/solutions/technologies/protein_electrophoresis_blotting_and_imaging/protein_electrophoresis/technology_detail/pet12_img1.jpg" alt="Components of a vertical electrophoresis cell" /></p> <p class="caption"><strong>Components of a vertical electrophoresis cell.</strong></p> <p>Vertical electrophoresis cells are manufactured in different size formats to accommodate different gels sizes. Cell selection is dependent on the requirements for speed, resolution, and throughput (both number of samples and number of gels) as well as the volume of sample available (see Table below).</p> <p><strong>Vertical Electrophoresis System Selection Guide</strong></p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td class="pd_tableheader pd_centeredcell" colspan="2"><strong><a href="http://www.bio-rad.com/en-us/product/electrophoresis-chambers/mini-protean-tetra-cell">Mini-PROTEAN<sup>&reg;</sup> System</a></strong></td> <td class="pd_tableheader pd_centeredcell"><strong><a href="http://www.bio-rad.com/en-us/product/electrophoresis-chambers/criterion-cell">Criterion&trade; System</a></strong></td> <td class="pd_tableheader pd_centeredcell"><strong><a href="http://www.bio-rad.com/en-us/product/large-format-1-d-electrophoresis-chambers/protean-ii-xi-cell">PROTEAN<sup>&reg;</sup> II System</a></strong></td> <td class="pd_tableheader pd_centeredcell"><strong><a href="http://www.bio-rad.com/en-us/product/large-format-1-d-electrophoresis-chambers/protean-plus-dodeca-cell">PROTEAN<sup>&reg;</sup> Plus System</a></strong></td> </tr> <tr> <td class="pd_centeredcell" colspan="2"><img src="/webroot/web/images/lsr/products/electrophoresis/category_overlay_content/global/precast_img_01.jpg" border="0" alt="" width="150" /></td> <td class="pd_centeredcell"><img src="/webroot/web/images/lsr/products/electrophoresis/category_overlay_content/global/precast_img_02.jpg" border="0" alt="" width="135" /></td> <td class="pd_centeredcell"><img src="/webroot/web/images/lsr/products/electrophoresis/category_overlay_content/global/precast_img_03.jpg" border="0" alt="" width="145" /></td> <td class="pd_centeredcell"><img src="/webroot/web/images/lsr/products/electrophoresis/category_thumb/global/protean-plus-dodeca-cell-thumb-large-format-1-D-electrophoresis-chambers.jpg" alt="" width="153" height="98" /></td> </tr> <tr class="pd_colorbackground" valign="top"> <td colspan="5"><strong>Advantages:</strong></td> </tr> <tr class="pd_colorbackground" valign="top"> <td colspan="2">Run 1&ndash;4 precast or handcast gels in the Mini-PROTEAN<sup>&reg;</sup> Tetra cell up to 12 gels in the Mini-PROTEAN<sup>&reg;</sup> Dodeca cell in mini format<br /><br /> Wing clamp assembly allows faster setup and leak-free operation <p>&nbsp;</p> </td> <td>Fast setup with drop-in gel and cell design (precast or handcast)<br /><br /> Run 1&ndash;2 Criterion&trade; precast or handcast gels<br /><br /> Integrated upper buffer chamber allows leak-free operation</td> <td>Large format gel system offers greater resolution over smaller formats and can be used with handcast or precast gels<br /><br /> Can accommodate up to 4 gels and is available in xi or XL format for running a variety of gel sizes<br /><br /> Multi-cell is available for running up to 6 gels</td> <td>Offers maximum resolution in a single gel and the longest range of separation (with the ability to run up to 12 gels)<br /><br /> Specifically for the second dimension of 2-d electrophoresis<br /><br /></td> </tr> <tr valign="top"> <td colspan="5"><strong>Compatible Gel Formats</strong></td> </tr> <tr valign="top"> <td>Precast</td> <td><a href="http://www.bio-rad.com/en-us/category/protein-gels/mini-protean-precast-gels">Mini-PROTEAN Precast Gels</a><br /><br /> <a href="http://www.bio-rad.com/en-us/category/protein-gels/ready-gel-precast-gels">Ready Gel<sup>&reg;</sup> Precast Gels</a></td> <td><a href="http://www.bio-rad.com/en-us/category/protein-gels/criterion-precast-gels">Criterion Precast Gels</a></td> <td>&nbsp;</td> <td>&nbsp;</td> </tr> <tr valign="top"> <td>Handcast</td> <td><a href="/en-us/product/electrophoresis-chambers/mini-protean-tetra-handcast-systems?tab=Plates">Mini-PROTEAN Empty Cassettes and Casting Plates</a><br /></td> <td><a href="/en-us/product/electrophoresis-chambers/criterion-cell?tab=Accessories">Criterion Empty Cassettes</a></td> <td><a href="/en-us/product/large-format-1-d-electrophoresis-chambers/protean-ii-xi-cell?tab=Accessories">PROTEAN II Casting Plates</a></td> <td><a href="/en-us/product/large-format-1-d-electrophoresis-chambers/protean-plus-hinged-spacer-plates-combs">PROTEAN Plus casting equipment</a></td> </tr> <tr class="pd_colorbackground" valign="top"> <td colspan="5"><strong>Electrophoresis Cells</strong></td> </tr> <tr class="pd_colorbackground" valign="top"> <td colspan="2"><a href="http://www.bio-rad.com/en-us/product/electrophoresis-chambers/mini-protean-tetra-cell">Mini-PROTEAN Tetra Cell</a>&nbsp; <br /><br /><a href="http://www.bio-rad.com/en-us/product/electrophoresis-chambers/mini-protean-3-dodeca-cell">Mini-PROTEAN<sup>&reg;</sup> 3 Dodeca</a><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=f9e3876d-3d8b-4e4c-965d-bc8a17dfc3d5">&trade; Cell<br /></a></td> <td><a href="http://www.bio-rad.com/en-us/product/electrophoresis-chambers/criterion-cell">Criterion Cell</a><br /><br /> <p><a href="http://www.bio-rad.com/en-us/product/electrophoresis-chambers/criterion-dodeca-cell">Criterion&trade; Dodeca</a><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=f9e3876d-3d8b-4e4c-965d-bc8a17dfc3d5">&trade;</a><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=f9e3876d-3d8b-4e4c-965d-bc8a17dfc3d5"> Cell<br /></a></p> </td> <td><a href="http://www.bio-rad.com/en-us/product/large-format-1-d-electrophoresis-chambers/protean-ii-xi-cell">PROTEAN II xi/XL Cell</a><br /><br /> <a href="http://www.bio-rad.com/en-us/product/large-format-1-d-electrophoresis-chambers/protean-ii-xi-xl-multi-cells">PROTEAN II xi/XL Multi-Cells</a></td> <td><a href="http://www.bio-rad.com/en-us/product/large-format-1-d-electrophoresis-chambers/protean-plus-dodeca-cell">PROTEAN Plus Dodeca</a></td> </tr> <tr> <td colspan="5"><strong>Precast Gel Dimensions</strong></td> </tr> <tr valign="top"> <td>(W x L x<br /> thickness)</td> <td>Mini-PROTEAN Precast Gels: <br /> 8.6 x 6.7 x 0.1 cm<br /><br /> Ready Gel Precast Gels: <br /> 8.3 x 6.4 x 0.1 cm</td> <td>Criterion Precast Gels:<br /> 13.3 x 8.7 x 0.1 cm</td> <td>&nbsp;</td> <td>&nbsp;</td> </tr> <tr class="pd_colorbackground" valign="top"> <td colspan="5"><strong>Cassette Dimensions (for Handcasting Gels)</strong></td> </tr> <tr class="pd_colorbackground" valign="top"> <td colspan="2">10.0 x 8.0 cm</td> <td>15.0 x 10.6 cm</td> <td>20.0 x 18.3 cm</td> <td>18.5 x 20.5 cm<br /> 20.0 x 20.5 cm <br /> 20.0 x 20.5 cm <br /></td> </tr> <tr valign="top"> <td colspan="5"><strong>Compatible Transfer Systems</strong></td> </tr> <tr valign="top"> <td>Wet/tank<br /> transfer</td> <td><a href="http://www.bio-rad.com/en-us/product/mini-trans-blot-cell">Mini Trans-Blot<sup>&reg;</sup> Cell</a> <br /><br /> <a href="http://www.bio-rad.com/en-us/product/criterion-blotter">Criterion Blotter </a><br /><br /> <a href="http://www.bio-rad.com/en-us/product/trans-blot-cell">Trans-Blot Cell</a></td> <td><a href="/en-us/product/criterion-blotter">Criterion Blotter</a><br /><br /> <a href="http://www.bio-rad.com/en-us/product/trans-blot-cell">Trans-Blot Cell </a><br /><br /> <a href="http://www.bio-rad.com/en-us/product/trans-blot-plus-cell">Trans-Blot Plus Cell</a></td> <td><a href="http://www.bio-rad.com/en-us/product/trans-blot-cell">Trans-Blot Cell </a><br /><br /> <a href="http://www.bio-rad.com/en-us/product/trans-blot-plus-cell">Trans-Blot Plus Cell</a></td> <td><a href="http://www.bio-rad.com/en-us/product/trans-blot-plus-cell">Trans-Blot Plus Cell </a></td> </tr> <tr valign="top"> <td>Semi-dry<br /> transfer</td> <td><a href="http://www.bio-rad.com/en-us/product/trans-blot-sd-semi-dry-transfer-cell">Trans-Blot SD Cell </a><br /><br /> <a href="/en-us/product/semi-dry-rapid-blotting-systems/trans-blot-turbo-transfer-system">Trans-Blot<sup>&reg;</sup> Turbo&trade; Transfer System</a></td> <td><a href="http://www.bio-rad.com/en-us/product/trans-blot-sd-semi-dry-transfer-cell">Trans-Blot SD Cell</a><br /><br /> <a href="/en-us/product/semi-dry-rapid-blotting-systems/trans-blot-turbo-transfer-system">Trans-Blot<sup>&reg;</sup> Turbo&trade; Transfer System</a></td> <td><a href="http://www.bio-rad.com/en-us/product/trans-blot-sd-semi-dry-transfer-cell">Trans-Blot SD Cell</a></td> <td>&nbsp;</td> </tr> </tbody> </table> <p><strong>Vertical Electrophoresis System Selection Guide</strong></p> <ul> <li><strong> Mini-format systems</strong> &mdash; accommodate small gels (up to 8.6 x 6.7 cm). The short separation distance maximizes the electrical field strength (V/cm) to yield rapid separations with moderate resolution. Use these systems for rapid analysis, method development, or when sample volumes are limited. The Mini-PROTEAN<sup>&reg;</sup> system includes the <a href="http://www.bio-rad.com/en-us/product/electrophoresis-chambers/mini-protean-tetra-cell">Mini-PROTEAN Tetra cell</a> (with a capacity of up to four gels) and the high-throughput <a href="http://www.bio-rad.com/en-us/product/electrophoresis-chambers/mini-protean-3-dodeca-cell">Mini-PROTEAN 3 Dodeca cell</a> (for running up to 12 gels)</li> <li><strong>Midi-format systems</strong> &mdash; accommodate 13.3 x 8.7 cm gels and offer rapid runs with higher sample capacity and enhanced separation over mini-format gels. The Criterion&trade; system includes the <a href="/en-us/product/electrophoresis-chambers/criterion-cell">Criterion Cell</a> (for 1&ndash;2 gels) and high-throughput <a href="http://www.bio-rad.com/en-us/product/electrophoresis-chambers/criterion-dodeca-cell">Criterion Dodeca cell</a> (for 1&ndash;12 gels); both cells are compatible with Criterion precast gels</li> <li><strong>Large-format systems</strong> &mdash; accommodate large gels (up to 20.0 x 18.3 cm for the PROTEAN<sup>&reg;</sup> II system, designed for one-dimensional PAGE) and so offer maximum resolution. The PROTEAN II system provides a choice of glass plates, spacer, and sandwich clamps to cast two gel lengths/ 10 or 20 cm. The PROTEAN<sup>&reg;</sup> Plus Dodeca&trade; cell allows maximum throughput with the capability to run up to 12 2-D gels at a time </li> </ul> <div class="top"><a href="#helptop">Back to Top</a></div> <p><a id="power_supplies_for_PAGE_apps" name="power_supplies_for_PAGE_apps"></a></p> Power Supplies for PAGE Applications <p>Power supplies are available to meet the power requirements of numerous applications (see below). The choice of power supply for PAGE applications usually depends on the size and number of gels being run. The following Table compares the Bio-Rad PowerPac&trade; power supplies recommended for vertical electrophoresis applications.</p> <ul> <li>For mini-format vertical PAGE applications, the <a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=bea5dea1-cef0-43ad-8af5-b2c0287f6e07">PowerPac Basic</a> and <a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=65e1d658-c899-4011-a7eb-406797c3c9e8">PowerPac HC</a> power supply are both suitable</li> <li>For large-format vertical PAGE applications, the <a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=b64403c7-43a2-4085-aba0-ce78c2b6f330">PowerPac HV</a> and <a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=89cb0329-d728-4689-87e8-47d77fd0a9f0">PowerPac Universal</a> power supply are both suitable</li> <li>For applications that require high currents, such as PAGE with the high-throughput Dodeca cells, the PowerPac HC power supply is the appropriate model</li> </ul> <p><strong>PowerPac power supplies selection guide</strong></p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td rowspan="2"><strong>Technique and Recommended Apparatus</strong></td> <td colspan="2"><strong>Typical Conditions* (Initial)</strong></td> <td colspan="2"><strong>Typical Conditions* (Final)</strong></td> <td rowspan="2"><strong>Run Time</strong></td> <td rowspan="2"><strong>PowerPac&trade; Power Supply</strong></td> </tr> <tr> <td><strong>V</strong></td> <td><strong>mA</strong></td> <td><strong>V</strong></td> <td><strong>mA</strong></td> </tr> <tr class="pd_colorbackground"> <td colspan="10"><strong>Laemmli (SDS), O'Farrell Second Dimension (SDS)</strong></td> </tr> <tr class="pd_colorbackground"> <td>PROTEAN<sup>&reg;</sup> II xi cell</td> <td>100</td> <td>35(C)</td> <td>350</td> <td>35(C)</td> <td>5 hr</td> <td>HV or Universal</td> </tr> <tr class="pd_colorbackground"> <td>PROTEAN II XL cell</td> <td>100</td> <td>35(C)</td> <td>350</td> <td>35(C)</td> <td>5 hr</td> <td>HV or Universal</td> </tr> <tr class="pd_colorbackground"> <td>Criterion&trade; cell</td> <td>200(C)</td> <td>200</td> <td>200(C)</td> <td>80</td> <td>55 min</td> <td>Basic or HC</td> </tr> <tr class="pd_colorbackground"> <td>Mini-PROTEAN<sup>&reg;</sup> Tetra cell</td> <td>200(C)</td> <td>240</td> <td>200(C)</td> <td>120</td> <td>35&ndash;45 min</td> <td>Basic or HC</td> </tr> <tr> <td colspan="10"><strong>High-Throughput Electrophoresis</strong></td> </tr> <tr> <td>PROTEAN Plus</td> <td>200(C)</td> <td>1,000</td> <td>200(C)</td> <td>350</td> <td>6 hr</td> <td>HC or Universal</td> </tr> <tr> <td rowspan="2">Dodeca&trade; cell</td> <td>200(C)</td> <td>1,000</td> <td>200(C)</td> <td>350</td> <td>6 hr</td> <td>HC or Universal</td> </tr> <tr> <td>150(C)</td> <td>1,200</td> <td>150(C)</td> <td>300</td> <td>18&ndash;20 hr</td> <td>HC or Universal</td> </tr> <tr> <td>PROTEAN II xi/XL multi-cell</td> <td>150</td> <td>480(C)</td> <td>500</td> <td>480(C)</td> <td>5 hr</td> <td>Universal</td> </tr> <tr> <td>Criterion&trade; Dodeca&trade; cell</td> <td>200(C)</td> <td>1,000&ndash;1,400</td> <td>200(C)</td> <td>400&ndash;500</td> <td>55 min</td> <td>HC or Universal</td> </tr> <tr> <td>Mini-PROTEAN<sup>&reg;</sup> 3 Dodeca&trade; cell</td> <td>200(C)</td> <td>600</td> <td>200(C)</td> <td>360</td> <td>45 min</td> <td>HC or Universal</td> </tr> <tr class="pd_colorbackground"> <td colspan="10"><strong>Western Blotting</strong></td> </tr> <tr class="pd_colorbackground"> <td>Mini Trans-Blot<sup>&reg;</sup> cell</td> <td>100(C)</td> <td>250</td> <td>100(C)</td> <td>450</td> <td>1 hr</td> <td>HC</td> </tr> <tr class="pd_colorbackground"> <td colspan="10">Criterion&trade; blotter</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;Wire electrodes</td> <td>100(C)</td> <td>250</td> <td>100(C)</td> <td>450</td> <td>1 hr</td> <td>HC</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;Plate electrodes</td> <td>100(C)</td> <td>650</td> <td>100(C)</td> <td>1,600</td> <td>30 min</td> <td>HC</td> </tr> <tr class="pd_colorbackground"> <td colspan="10">Trans-Blot<sup>&reg;</sup> cell</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;Wire electrodes</td> <td>60(C)</td> <td>210&ndash;250</td> <td>60(C)</td> <td>210&mdash;250</td> <td>5 hr</td> <td>HC</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;Plate electrodes</td> <td>100&ndash;150(C)</td> <td>1,000&ndash;1,600</td> <td>100&ndash;150(C)</td> <td>1,000&ndash;1,600</td> <td>1&ndash;5 hr</td> <td>HC</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;High-intensity transfer</td> <td>50&ndash;100(C)</td> <td>1,600</td> <td>50&ndash;100(C)</td> <td>1,600</td> <td>30 min</td> <td>HC</td> </tr> <tr class="pd_colorbackground"> <td>Trans-Blot<sup>&reg;</sup> Plus cell</td> <td>100(C)</td> <td>3,000</td> <td>100(C)</td> <td>3,000</td> <td>30 min&ndash;1 hr</td> <td>HC</td> </tr> <tr class="pd_colorbackground"> <td colspan="10">Trans-Blot<sup>&reg;</sup> SD cell</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;Protein</td> <td>15(C)</td> <td>500</td> <td>15(C)</td> <td>200</td> <td>15&ndash;30 min</td> <td>HC</td> </tr> <tr class="pd_colorbackground"> <td>&nbsp;&nbsp;DNA/RNA</td> <td>15</td> <td>650(C)</td> <td>25</td> <td>650(C)</td> <td>10&ndash;30 min</td> <td>HC</td> </tr> </tbody> </table> <p class="caption">*(C) = constant. Typical conditions are listed as guidelines only and can vary based on sample, buffers, etc.</p> <p><img src="/webroot/web/images/lsr/solutions/technologies/protein_electrophoresis_blotting_and_imaging/protein_electrophoresis/technology_detail/pet12_img2.jpg" alt="PowerPac power supplies" /></p> <p class="caption"><strong>PowerPac power supplies.</strong></p> <div class="top"><a href="#helptop">Back to Top</a></div> References <p>Andrews AT (1986). Electrophoresis: Theory, Techniques and Biochemical and Clinical Applications (New York: Oxford University Press).</p> <div class="top"><a href="#helptop">Back to Top</a></div> <div class="videowrap vwrap_last"> <div class="videoImg"><a title="Mini-PROTEAN Tetra Cell: Features, Parts, and Assembly" onclick="window.open('http://bio-rad.centra.com/bio-radroot/learning%20objects/mini-protean_tetra/mini-protean_tetra_cell.htm', 'StatusBar', 'toolbar=no, resizable=no, width=800, height=600');" href="javascript:void(0);"><img style="border:none;" src="https://www.bio-rad.com/webroot/web/images/lsr/support/tutorials/global/mini-protean.png" alt="" /></a></div> <div class="videoDesc"><a title="Mini-PROTEAN Tetra Cell: Features, Parts, and Assembly" onclick="window.open('http://bio-rad.centra.com/bio-radroot/learning%20objects/mini-protean_tetra/mini-protean_tetra_cell.htm', 'StatusBar', 'toolbar=no, resizable=no, width=800, height=600');" href="javascript:void(0);">Mini-PROTEAN<sup>&reg;</sup> Tetra Cell: Features, Parts, and Assembly</a><br /> This tutorial highlights the main features and components of the Mini-PROTEAN Tetra cell and shows you how to assemble the unit and its handcasting accessories. Information about the compatibility of its components with the Mini-PROTEAN 3 and Mini Trans-Blot<sup>&reg;</sup> cells is also provided.</div> <div class="clear">&nbsp;</div> </div> <div class="videowrap vwrap_last"> <div class="videoImg"><a title="Bio-Rad's PowerPac Family of Power Supplies" onclick="window.open('/webroot/web/html/lsr/support/lsr_electro_powerpac_supply.html', 'StatusBar', 'toolbar=no, resizable=no, width=800, height=600');" href="javascript:void(0);"><img style="border:none;" src="https://www.bio-rad.com/webroot/web/images/lsr/support/tutorials/global/thb_powerpacs.png" alt="" /></a></div> <div class="videoDesc"><a title="Bio-Rad's PowerPac Family of Power Supplies" onclick="window.open('/webroot/web/html/lsr/support/lsr_electro_powerpac_supply.html', 'StatusBar', 'toolbar=no, resizable=no, width=800, height=600');" href="javascript:void(0);">Bio-Rad's PowerPac&trade; Family of Power Supplies</a><br /> This tutorial describes the features of Bio-Rad's PowerPac family of power supplies and compares their specifications. It also offers information about parameter selection, common error messages, and available PowerPac accessories. Important safety information is also provided.</div> <div class="clear">&nbsp;</div> </div> 6040 /templatedata/internet/documentation/data/LSR/Literature/6040.xml 5535 2710 1760 2881 2882 2885 3189 Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/2D-Electrophoresis/2nd Dimension Mini Format Electrophoresis Systems/Mini-PROTEAN Tetra Cell Systems ->MT::91d3ccf0-0657-4e53-be59-7b5446f6fb54##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Midi Format 1D-Electrophoresis Systems/Criterion Cell Systems ->MT::b94e30f5-0f2a-452d-a3a0-825e8205b476##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/2D-Electrophoresis/2nd Dimension Large Format Electrophoresis Systems/PROTEAN II xi Cells ->MT::15fb8e8f-b179-41d3-9222-6b96de3a0648##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/2D-Electrophoresis/1st Dimension- Isoelectric Focusing (IEF)/PROTEAN IEF Cell ->MT::92682b19-051c-464f-8a73-97cd256b225f##Life Science Research/Products/Electrophoresis and Blotting/Experion Automated Electrophoresis System/Experion Automated Electrophoresis Station ->MT::e88b061b-37dc-4610-b4b7-0ef925f622f9## Life Science Research/Solutions/Technologies/2-D Electrophoresis ->MTS::LUSQG6LPT##Life Science Research/Solutions/Technologies/Western Blotting ->MTS::LUSPPAKG4## Life Science Research/Solutions/Applications/Protein Separation and Analysis ->MTS::LUSOU3F7Q##Life Science Research/Solutions/Applications/2-D Electrophoresis and Analysis ->MTS::LUSQF04EH##Life Science Research/Solutions/Applications/Automated Electrophoresis and Analysis ->MTS::LUSQXB15##Life Science Research/Solutions/Technologies/Automated Electrophoresis System -Experion- ->MTS::LUSR04KG4## Eddie C Protein Electrophoresis Equipment 12/14/11 03:30 PM 12/14/21 03:32 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Technologies/Protein_Electrophoresis N 0
Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.